Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit

Description

• The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng)
• It contains all required components to perform cell lysis, reverse transcription (RT), and PCR amplification in a convenient premixed format
• A combination of superior enzymes (SuperScript IV Reverse Transcriptase and Platinum SuperFi U DNA Polymerase) enables high yields, sensitivity, and accuracy
• Due to the short reaction times and minimal hands-on time, this simple one-tube preamplification protocol can be completed in just over two hours
• The high quality global cDNA obtained can be used for next-generation sequencing (NGS) applications or for gene expression analysis by real-time PCR
• Features of the SuperScript IV Single Cell/Low Input cDNA PreAmp Kit include: • Superior sensitivity for easy detection of low abundant targets (as low as single cell or 2 pg total RNA) • High quality cDNA for full-length transcript information with uniform coverage • Streamlined workflow with simple one-tube protocol • Short lysis and reverse transcription times for time savings • Global preamplification compatible with downstream analysis by NGS or real-time PCR The SuperScript IV Single Cell/Low Input cDNA PreAmp Kit leverages the terminal deoxynucleotidyl transferase (TdT) activity of the SuperScript IV Reverse Transcriptase
• Capturing oligonucleotide containing oligo(dT) and adapter sequences is used as primer for selective first strand cDNA synthesis from poly(A)-containing RNAs
• When the reverse transcriptase reaches the 5‘ end of the RNA template, TdT activity adds 1–3 extra nucleotides to the cDNA end, enabling template switching and ligation-free incorporation of adapter sequence in the 3‘ end of the resulting cDNA
• The high sensitivity and template switching efficiency of SuperScript IV Reverse Transcriptase allow the capture of even low-abundance targets
• Adapter sequences incorporated in both ends of the cDNA serve as primer-binding sites in the subsequent PCR amplification step, allowing global preamplification of the full-length templates
• The included Platinum SuperFi U Preamplification Master Mix contains a superior high-fidelity DNA polymerase that ensures efficient amplification of long templates (up to 10 kb) with no PCR-induced bias or errors.