11-752-096
Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-752-096-Each-of-1 | In Stock | ₹ 1,98,927.00 |
11-752-096 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,98,927.00
GST (18%): ₹ 35,806.86
Total Price: ₹ 2,34,733.86
Content And Storage
Two packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
GC-Rich PCR Performance
High
Reaction Speed
30 min.
Optimal Reaction Temperature
50°C
Shipping Condition
Dry Ice
Final Product Type
PCR Amplified cDNA
Reagent Type
Reverse Transcription
Starting Material
Cells or RNA
Format
Kit
Polymerase
Platinum SuperFi U DNA Polymerase
Technique
Reverse Transcription, Preamplification
Reverse Transcriptase
SuperScript IV
For Use With (Application)
Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions
96 Reactions
Size (Final Product)
Up to 10 kb
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Description
- The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng)
- It contains all required components to perform cell lysis, reverse transcription (RT), and PCR amplification in a convenient premixed format
- A combination of superior enzymes (SuperScript IV Reverse Transcriptase and Platinum SuperFi U DNA Polymerase) enables high yields, sensitivity, and accuracy
- Due to the short reaction times and minimal hands-on time, this simple one-tube preamplification protocol can be completed in just over two hours
- The high quality global cDNA obtained can be used for next-generation sequencing (NGS) applications or for gene expression analysis by real-time PCR
- Features of the SuperScript IV Single Cell/Low Input cDNA PreAmp Kit include: • Superior sensitivity for easy detection of low abundant targets (as low as single cell or 2 pg total RNA) • High quality cDNA for full-length transcript information with uniform coverage • Streamlined workflow with simple one-tube protocol • Short lysis and reverse transcription times for time savings • Global preamplification compatible with downstream analysis by NGS or real-time PCR The SuperScript IV Single Cell/Low Input cDNA PreAmp Kit leverages the terminal deoxynucleotidyl transferase (TdT) activity of the SuperScript IV Reverse Transcriptase
- Capturing oligonucleotide containing oligo(dT) and adapter sequences is used as primer for selective first strand cDNA synthesis from poly(A)-containing RNAs
- When the reverse transcriptase reaches the 5‘ end of the RNA template, TdT activity adds 1–3 extra nucleotides to the cDNA end, enabling template switching and ligation-free incorporation of adapter sequence in the 3‘ end of the resulting cDNA
- The high sensitivity and template switching efficiency of SuperScript IV Reverse Transcriptase allow the capture of even low-abundance targets
- Adapter sequences incorporated in both ends of the cDNA serve as primer-binding sites in the subsequent PCR amplification step, allowing global preamplification of the full-length templates
- The included Platinum SuperFi U Preamplification Master Mix contains a superior high-fidelity DNA polymerase that ensures efficient amplification of long templates (up to 10 kb) with no PCR-induced bias or errors.
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Content And Storage: Two packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
GC-Rich PCR Performance: High
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Shipping Condition: Dry Ice
Final Product Type: PCR Amplified cDNA
Reagent Type: Reverse Transcription
Starting Material: Cells or RNA
Format: Kit
Polymerase: Platinum SuperFi U DNA Polymerase
Technique: Reverse Transcription, Preamplification
Reverse Transcriptase: SuperScript IV
For Use With (Application): Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions: 96 Reactions
Size (Final Product): Up to 10 kb
Content And Storage:
Two packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
GC-Rich PCR Performance:
High
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Shipping Condition:
Dry Ice
Final Product Type:
PCR Amplified cDNA
Reagent Type:
Reverse Transcription
Starting Material:
Cells or RNA
Format:
Kit
Polymerase:
Platinum SuperFi U DNA Polymerase
Technique:
Reverse Transcription, Preamplification
Reverse Transcriptase:
SuperScript IV
For Use With (Application):
Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions:
96 Reactions
Size (Final Product):
Up to 10 kb
Content And Storage: Four packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
GC-Rich PCR Performance: High
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Shipping Condition: Dry Ice
Final Product Type: PCR Amplified cDNA
Reagent Type: Reverse Transcription
Starting Material: Cells or RNA
Format: Kit
Polymerase: Platinum SuperFi U DNA Polymerase
Technique: Reverse Transcription, Preamplification
Reverse Transcriptase: SuperScript IV
For Use With (Application): Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions: 192 Reactions
Size (Final Product): Up to 10 kb
Content And Storage:
Four packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
GC-Rich PCR Performance:
High
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Shipping Condition:
Dry Ice
Final Product Type:
PCR Amplified cDNA
Reagent Type:
Reverse Transcription
Starting Material:
Cells or RNA
Format:
Kit
Polymerase:
Platinum SuperFi U DNA Polymerase
Technique:
Reverse Transcription, Preamplification
Reverse Transcriptase:
SuperScript IV
For Use With (Application):
Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions:
192 Reactions
Size (Final Product):
Up to 10 kb
Content And Storage: • 2X RT Reaction Mix (2 x 1.25 mL)• RT Enzyme Mix (500 μL)•E. coliRNase (250 μL)Sufficient reagents for 250 reactions at 20 μL reaction volumes.Store at –20°C.
GC-Rich PCR Performance: High
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Super Mix
Polymerase: __
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
For Use With (Application): Real Time PCR (qPCR)
No. of Reactions: 250 Reactions
Size (Final Product): __
Content And Storage:
• 2X RT Reaction Mix (2 x 1.25 mL)• RT Enzyme Mix (500 μL)•E. coliRNase (250 μL)Sufficient reagents for 250 reactions at 20 μL reaction volumes.Store at –20°C.
GC-Rich PCR Performance:
High
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Super Mix
Polymerase:
__
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
For Use With (Application):
Real Time PCR (qPCR)
No. of Reactions:
250 Reactions
Size (Final Product):
__
Content And Storage: Ten packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
GC-Rich PCR Performance: High
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Shipping Condition: Dry Ice
Final Product Type: PCR Amplified cDNA
Reagent Type: Reverse Transcription
Starting Material: Cells or RNA
Format: Kit
Polymerase: Platinum SuperFi U DNA Polymerase
Technique: Reverse Transcription, Preamplification
Reverse Transcriptase: SuperScript IV
For Use With (Application): Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions: 480 Reactions
Size (Final Product): Up to 10 kb
Content And Storage:
Ten packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
GC-Rich PCR Performance:
High
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Shipping Condition:
Dry Ice
Final Product Type:
PCR Amplified cDNA
Reagent Type:
Reverse Transcription
Starting Material:
Cells or RNA
Format:
Kit
Polymerase:
Platinum SuperFi U DNA Polymerase
Technique:
Reverse Transcription, Preamplification
Reverse Transcriptase:
SuperScript IV
For Use With (Application):
Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions:
480 Reactions
Size (Final Product):
Up to 10 kb