18-091-150
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-091-150-Each-of-1 | In Stock | ₹ 58,865.28 |
18-091-150 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 58,865.28
GST (18%): ₹ 10,595.75
Total Price: ₹ 69,461.03
Content And Storage
• Oligo(dT)20, 50 μL (50 μM)• Random hexamers, 250 μL (50 ng/μL)• 5X RT buffer, 1 mL• DTT, 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 50 μL (200 U/μL)• Ribonuclease Inhibitor, 100 μL (40 U/μL)•E. coliRNase H, 50 μL (2 U/μL)• DEPC-treated water, 1.2 mL• Total HeLa RNA, 20 μL (10 ng/μL)• Sense Control Primer, 25 μL (10 μM)• Antisense Control Primer, 25 μL (10 μM)• ezDNase Enzyme, 50 μL• 10X ezDNase Buffer, 100 μL• Nuclease-free water, 1.25 mLStore at -15°C to -25°C.
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Reverse Transcriptase
SuperScript IV
No. of Reactions
50 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Format
Kit
Reaction Speed
10 min.
Optimal Reaction Temperature
50°C
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Size (Final Product)
Up to 12.3 kb
Includes
Kit with ezDNase
Related Products
Description
- The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA
- The system includes SuperScript IV Reverse Transcriptase (RT), a proprietary MMLV mutant that produces robust and reliable cDNA synthesis in RT reactions
- The SuperScript IV First-Strand Synthesis System with ezDNase has the further ability to remove potential genomic DNA contamination
- The SuperScript IV synthesis system is significantly improved over the SuperScript III-based system in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of the previous system, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity
- The SuperScript IV system is designed to provide reliable, consistent, and fast cDNA synthesis in the presence of inhibitors found in a wide variety of samples that cause other currently available RTs to perform inefficiently
- The extremely simplified genomic DNA removal step of the SuperScript IV First-Strand Synthesis System with ezDNase dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment
- The SuperScript IV synthesis system with ezDNase is the top choice for performance and flexibility for RT-PCR applications. We recommend it for all RT-PCR applications, especially when reproducibility and reliability is the primary concern and when inhibitors in the RNA sample may interfere with cDNA synthesis, leading to biases in gene expression studies
- Both SuperScript IV First-Strand Synthesis systems contain all components needed for RT reactions, plus an additional control gene and primers, and provide the flexibility to customize the RT set-up to fit the needs of your application
- Features of the SuperScript IV First-Strand Synthesis System include: • Significantly improved resistance to a variety of inhibitors that can interfere with cDNA synthesis • Robust and specific cDNA synthesis in a wide range of sample types • A faster reverse transcriptase reaction that reduces incubation time from >50 min to 10 min • Significantly better processivity compared to SuperScript III RT • Simplified genomic DNA removal step (SuperScript IV synthesis system with ezDNase only) Enzyme source Purified from E
- coli expressing the pol gene of M-MLV, modified to increase thermostability, half-life, processivity, and inhibitor resistance and to reduce RNase H activity
- Performance and quality testing Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product Unit definition One unit of SuperScript IV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT) 12-18 as a template/primer
- Unit reaction conditions 50 mM Tris-HCl (pH 8.3), 4 mM MgCl 2 , 10 mM DTT, 50 mM KCl, 0.5 mM dTTP, 0.4 MBq/mL [ 3 H]-dTTP, 0.4 mM poly(A) oligo (dT) 12-18 and enzyme in 20 μL for 10 min at 37°C
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Content And Storage: • Oligo(dT)20, 50 μL (50 μM)• Random hexamers, 250 μL (50 ng/μL)• 5X RT buffer, 1 mL• DTT, 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 50 μL (200 U/μL)• Ribonuclease Inhibitor, 100 μL (40 U/μL)•E. coliRNase H, 50 μL (2 U/μL)• DEPC-treated water, 1.2 mL• Total HeLa RNA, 20 μL (10 ng/μL)• Sense Control Primer, 25 μL (10 μM)• Antisense Control Primer, 25 μL (10 μM)• ezDNase Enzyme, 50 μL• 10X ezDNase Buffer, 100 μL• Nuclease-free water, 1.25 mLStore at -15°C to -25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript IV
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Includes: Kit with ezDNase
Content And Storage:
• Oligo(dT)20, 50 μL (50 μM)• Random hexamers, 250 μL (50 ng/μL)• 5X RT buffer, 1 mL• DTT, 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 50 μL (200 U/μL)• Ribonuclease Inhibitor, 100 μL (40 U/μL)•E. coliRNase H, 50 μL (2 U/μL)• DEPC-treated water, 1.2 mL• Total HeLa RNA, 20 μL (10 ng/μL)• Sense Control Primer, 25 μL (10 μM)• Antisense Control Primer, 25 μL (10 μM)• ezDNase Enzyme, 50 μL• 10X ezDNase Buffer, 100 μL• Nuclease-free water, 1.25 mLStore at -15°C to -25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript IV
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
Includes:
Kit with ezDNase
Content And Storage: • Oligo(dT)20, 4 x 50 μL (50 μM)• Random hexamers, 4 x 250 μL (50 ng/μL)• 5X RT buffer, 4 x 1 mL• DTT, 4 x 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 4 x 50 μL (200 U/μL)• Ribonuclease Inhibitor, 4 x 100 μL (40 U/μL)•E. coliRNase H, 4 x 50 μL (2 U/μL)• DEPC-treated water, 4 x 1.2 mL• Total HeLa RNA, 4 x 20 μL (10 ng/μL)• Sense Control Primer, 4 x 25 μL (10 μM)• Antisense Control Primer, 4 x 25 μL (10 μM)Store at -15°C to -25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript IV
No. of Reactions: 200 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Includes: Kit only
Content And Storage:
• Oligo(dT)20, 4 x 50 μL (50 μM)• Random hexamers, 4 x 250 μL (50 ng/μL)• 5X RT buffer, 4 x 1 mL• DTT, 4 x 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 4 x 50 μL (200 U/μL)• Ribonuclease Inhibitor, 4 x 100 μL (40 U/μL)•E. coliRNase H, 4 x 50 μL (2 U/μL)• DEPC-treated water, 4 x 1.2 mL• Total HeLa RNA, 4 x 20 μL (10 ng/μL)• Sense Control Primer, 4 x 25 μL (10 μM)• Antisense Control Primer, 4 x 25 μL (10 μM)Store at -15°C to -25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript IV
No. of Reactions:
200 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
Includes:
Kit only
Content And Storage: • Oligo(dT)20, 4 x 50 μL (50 μM)• Random hexamers, 4 x 250 μL (50 ng/μL)• 5X RT buffer, 4 x 1 mL• DTT, 4 x 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 4 x 50 μL (10,000 units total at 200 U/μL)• Ribonuclease Inhibitor, 4 x 100 μL (40 U/μL)•E. coliRNase H, 4 x 50 μL (2 U/μL)• DEPC-treated water, 4 x 1.2 mL• Total HeLa RNA, 4 x 20 μL (10 ng/μL)• Sense Control Primer, 4 x 25 μL (10 μM)• Antisense Control Primer, 4 x 25 μL (10 μM)• ezDNase Enzyme, 4 x 50 μL• 10X ezDNase Buffer, 4 x 100 μL• Nuclease-free water, 4 x 1.25 mLStore at -15°C to -25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript IV
No. of Reactions: 200 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Includes: Kit with ezDNase
Content And Storage:
• Oligo(dT)20, 4 x 50 μL (50 μM)• Random hexamers, 4 x 250 μL (50 ng/μL)• 5X RT buffer, 4 x 1 mL• DTT, 4 x 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 4 x 50 μL (10,000 units total at 200 U/μL)• Ribonuclease Inhibitor, 4 x 100 μL (40 U/μL)•E. coliRNase H, 4 x 50 μL (2 U/μL)• DEPC-treated water, 4 x 1.2 mL• Total HeLa RNA, 4 x 20 μL (10 ng/μL)• Sense Control Primer, 4 x 25 μL (10 μM)• Antisense Control Primer, 4 x 25 μL (10 μM)• ezDNase Enzyme, 4 x 50 μL• 10X ezDNase Buffer, 4 x 100 μL• Nuclease-free water, 4 x 1.25 mLStore at -15°C to -25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript IV
No. of Reactions:
200 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
Includes:
Kit with ezDNase
Content And Storage: Each 2 ml plastic column contains 1 ml of Sephacryl™ S-500 HR resin. Store at +4°C.
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Format: Pre-packed Column
Reaction Speed: __
Optimal Reaction Temperature: __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Includes: __
Content And Storage:
Each 2 ml plastic column contains 1 ml of Sephacryl™ S-500 HR resin. Store at +4°C.
GC-Rich PCR Performance:
__
Technique:
__
Reverse Transcriptase:
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Format:
Pre-packed Column
Reaction Speed:
__
Optimal Reaction Temperature:
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Includes:
__