28-025-013
Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 28-025-013-Each-of-1 | In Stock | ₹ 33,731.00 |
28-025-013 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 33,731.00
GST (18%): ₹ 6,071.58
Total Price: ₹ 39,802.58
Content And Storage
• M-mLV RT, 200 μL (200 U/μL)• 5X First Strand Buffer, 1 mL• DTT, 500 μL (100 mM)Store at –20°C.
Reaction Speed
50 min.
Optimal Reaction Temperature
37°C
Ribonuclease H Activity
Yes
Concentration
200 U/μL
No. of Reactions
200 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Format
Stand-alone Enzyme
Technique
Reverse Transcription
Reverse Transcriptase
M-MLV
Shipping Condition
Wet Ice
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Size (Final Product)
Up to 7 kb
Related Products
Description
- M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid
- Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity
- One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT) 25 as template-primer
- Features of M-MLV Reverse Transcriptase include: • Excellent thermostability with optimal activity at 37°C • Can be used to synthesize first-strand cDNA up to 7 kb • Multiple applications including synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR Source Purified from E
- coli expressing the pol gene of M-MLV on a plasmid Performance and quality testing SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product Unit reaction conditions 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl 2 , 1 mM DTT, 0.5 mM [ 3 H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT) 25 , 0.1 mg/mL BSA, and enzyme in 50 μL for 10 min at 37°C Order Info Shipping Condition: Wet ice
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Content And Storage: • M-mLV RT, 200 μL (200 U/μL)• 5X First Strand Buffer, 1 mL• DTT, 500 μL (100 mM)Store at –20°C.
Reaction Speed: 50 min.
Optimal Reaction Temperature: 37°C
Ribonuclease H Activity: Yes
Concentration: 200 U/μL
No. of Reactions: 200 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Stand-alone Enzyme
Technique: Reverse Transcription
Reverse Transcriptase: M-MLV
Shipping Condition: Wet Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 7 kb
Content And Storage:
• M-mLV RT, 200 μL (200 U/μL)• 5X First Strand Buffer, 1 mL• DTT, 500 μL (100 mM)Store at –20°C.
Reaction Speed:
50 min.
Optimal Reaction Temperature:
37°C
Ribonuclease H Activity:
Yes
Concentration:
200 U/μL
No. of Reactions:
200 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Stand-alone Enzyme
Technique:
Reverse Transcription
Reverse Transcriptase:
M-MLV
Shipping Condition:
Wet Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 7 kb
Content And Storage: • M-mLV RT, 1 mL (200 U/μL)• First Strand Buffer, 4 x 1 mL• DTT, 4 x 500 μL (100 mM)Store at –20°C.
Reaction Speed: 50 min.
Optimal Reaction Temperature: 37°C
Ribonuclease H Activity: Yes
Concentration: 200 U/μL
No. of Reactions: 1,000 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Stand-alone Enzyme
Technique: Reverse Transcription
Reverse Transcriptase: M-MLV
Shipping Condition: Wet Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 7 kb
Content And Storage:
• M-mLV RT, 1 mL (200 U/μL)• First Strand Buffer, 4 x 1 mL• DTT, 4 x 500 μL (100 mM)Store at –20°C.
Reaction Speed:
50 min.
Optimal Reaction Temperature:
37°C
Ribonuclease H Activity:
Yes
Concentration:
200 U/μL
No. of Reactions:
1,000 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Stand-alone Enzyme
Technique:
Reverse Transcription
Reverse Transcriptase:
M-MLV
Shipping Condition:
Wet Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 7 kb
Content And Storage: Store in refrigerator (2°C to 8°C).
Reaction Speed: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
Concentration: __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Format: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Content And Storage:
Store in refrigerator (2°C to 8°C).
Reaction Speed:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
Concentration:
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Format:
__
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
Approved for shipment at Room Temperature or on Wet Ice
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Content And Storage: Store in refrigerator (2°C to 8°C).
Reaction Speed: __
Optimal Reaction Temperature: __
Ribonuclease H Activity: __
Concentration: __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Format: __
Technique: __
Reverse Transcriptase: __
Shipping Condition: Approved for shipment at Room Temperature or on Wet Ice
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Content And Storage:
Store in refrigerator (2°C to 8°C).
Reaction Speed:
__
Optimal Reaction Temperature:
__
Ribonuclease H Activity:
__
Concentration:
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Format:
__
Technique:
__
Reverse Transcriptase:
__
Shipping Condition:
Approved for shipment at Room Temperature or on Wet Ice
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__