Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)

Description

• M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid
• Compared to AMV RT, Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) lacks DNA endonuclease activity and has a lower RNase H activity
• One unit of M-MLV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT) 25 as template-primer
• Features of M-MLV Reverse Transcriptase include: • Excellent thermostability with optimal activity at 37°C • Can be used to synthesize first-strand cDNA up to 7 kb • Multiple applications including synthesis of first-strand cDNA, primer extension, sequencing dsDNA, cDNA libraries, and RT-PCR Source Purified from E
• coli expressing the pol gene of M-MLV on a plasmid Performance and quality testing SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product Unit reaction conditions 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl 2 , 1 mM DTT, 0.5 mM [ 3 H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT) 25 , 0.1 mg/mL BSA, and enzyme in 50 μL for 10 min at 37°C Order Info Shipping Condition: Wet ice