44-606-26
Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kits
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 44-606-26-Each-of-1 | In Stock | ₹ 4,19,244.00 |
44-606-26 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,19,244.00
GST (18%): ₹ 75,463.92
Total Price: ₹ 4,94,707.92
Content And Storage
MycoSEQ Mycoplasma Detection Kit• Power SYBR Green PCR Master Mix (2X), store at -15°C to -25°C on receipt, 2°C to 8°C after first use, protected from light• Mycoplasma Real-Time PCR Primer Mix (10X), -15°C to -25°C on receipt, 2°C to 8°C after first use• Discriminatory Positive/Extraction Control, -15°C to -25°C• Negative control, -15°C to -25°C on receipt, 2°C to 8°C after first usePrepSEQ 1-2-3 Mycoplasma Nucleic Acid Sample Extraction Kit• Cell Fractionation Buffer, store at 2°C to 8°C• RNase Cocktail Enzyme Mix, -15°C to -25°C• Lysis Buffer, 18°C to 25°C• Wash Buffer Concentrate, 18°C to 25°C• Elution Buffer, 18°C to 25°C• Proteinase K Buffer, 18°C to 25°C• Magnetic Particles, 18°C to 25°C• Proteinase K, -15°C to -25°C
For Use With
QS5 0.1 mL Block or 7500 FAST
Product Line
MycoSEQ™
Quantity
100 Reactions
Description
Detection for BioProduction process media, cells, raw materials
Label or Dye
SYBR Green
Product Type
Assay Kit and Sample Preparation
For Use With (Application)
Mycoplasma Detection
Related Products
Description
- The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT)
- The PCR primers were designed for mycoplasma detection using a proprietary bioinformatics pipeline to detect >90 mycoplasma species with high specificity and no cross-reactivity to closely-related bacterial species
- The assay's sensitivity has been demonstrated by internal and external validations to detect 10 CFU/mL or the genomic equivalent as recommended by regulatory guidance
- Features of the MycoSEQ Mycoplasma Detection Kit include: • Easy to use, results in less than one day • Includes a unique discriminatory positive control to differentiate a true positive result from a potential cross-contamination positive call • Includes protocols for various methods, including lot release and in-process testing • Live mycoplasma is not needed: MycoSEQ Mycoplasma Detection Kit does not contain any live mycoplasma and does not require use of live mycoplasma for validation • Can be used with gDNA, inactivated mycoplasma, or live mycoplasma • May be used as an alternative to the standard 28-day culture test • Can be used in conjunction with culture-based methods by providing preliminary results while waiting for results from the 28-day test The PrepSEQ nucleic acid extraction module included in MycoSEQ Mycoplasma Detection Kit, with PrepSEQ sample preparation (Cat
- No
- 4460626) offers a robust and easy workflow to overcome complex matrix challenges to ensure the required sensitivity
- For other recommended sample prep options, see the Integrated workflow solution section
- Also available but not included with the detection kit: MycoSEQ gDNA kits (Cat
- No
- A49973 and A49976), containing purified genomic DNA from various mycoplasma species, may be used as positive test material during method development
- Integrated workflow solution The MycoSEQ Mycoplasma Detection Kit is part of an integrated workflow for adventitious agent, impurity and contaminant testing during biopharmaceutical manufacturing
- The entire workflow, including PrepSEQ Sample Preparation Kits together with qPCR analysis, can provide results in under 5 hours
- (Please note complex matrices may require additional upfront processing steps, as described in various available protocols.) MycoSEQ kit performance has been tested and confirmed on the Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems QuantStudio 5 Real-Time PCR System
- Data analysis using AccuSEQ Real-Time PCR Detection Software provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance.
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Content And Storage: MycoSEQ Mycoplasma Detection Kit• Power SYBR Green PCR Master Mix (2X), store at -15°C to -25°C on receipt, 2°C to 8°C after first use, protected from light• Mycoplasma Real-Time PCR Primer Mix (10X), -15°C to -25°C on receipt, 2°C to 8°C after first use• Discriminatory Positive/Extraction Control, -15°C to -25°C• Negative control, -15°C to -25°C on receipt, 2°C to 8°C after first usePrepSEQ 1-2-3 Mycoplasma Nucleic Acid Sample Extraction Kit• Cell Fractionation Buffer, store at 2°C to 8°C• RNase Cocktail Enzyme Mix, -15°C to -25°C• Lysis Buffer, 18°C to 25°C• Wash Buffer Concentrate, 18°C to 25°C• Elution Buffer, 18°C to 25°C• Proteinase K Buffer, 18°C to 25°C• Magnetic Particles, 18°C to 25°C• Proteinase K, -15°C to -25°C
For Use With: QS5 0.1 mL Block or 7500 FAST
Product Line: MycoSEQ™
Quantity: 100 Reactions
Description: Detection for BioProduction process media, cells, raw materials
Label or Dye: SYBR Green
Product Type: Assay Kit and Sample Preparation
For Use With (Application): Mycoplasma Detection
Content And Storage:
MycoSEQ Mycoplasma Detection Kit• Power SYBR Green PCR Master Mix (2X), store at -15°C to -25°C on receipt, 2°C to 8°C after first use, protected from light• Mycoplasma Real-Time PCR Primer Mix (10X), -15°C to -25°C on receipt, 2°C to 8°C after first use• Discriminatory Positive/Extraction Control, -15°C to -25°C• Negative control, -15°C to -25°C on receipt, 2°C to 8°C after first usePrepSEQ 1-2-3 Mycoplasma Nucleic Acid Sample Extraction Kit• Cell Fractionation Buffer, store at 2°C to 8°C• RNase Cocktail Enzyme Mix, -15°C to -25°C• Lysis Buffer, 18°C to 25°C• Wash Buffer Concentrate, 18°C to 25°C• Elution Buffer, 18°C to 25°C• Proteinase K Buffer, 18°C to 25°C• Magnetic Particles, 18°C to 25°C• Proteinase K, -15°C to -25°C
For Use With:
QS5 0.1 mL Block or 7500 FAST
Product Line:
MycoSEQ™
Quantity:
100 Reactions
Description:
Detection for BioProduction process media, cells, raw materials
Label or Dye:
SYBR Green
Product Type:
Assay Kit and Sample Preparation
For Use With (Application):
Mycoplasma Detection
Content And Storage: 4°C short term, -80°C long term
For Use With: __
Product Line: __
Quantity: 100 μg
Description: __
Label or Dye: __
Product Type: Antibody
For Use With (Application): __
Content And Storage:
4°C short term, -80°C long term
For Use With:
__
Product Line:
__
Quantity:
100 μg
Description:
__
Label or Dye:
__
Product Type:
Antibody
For Use With (Application):
__
Content And Storage: -20°C
For Use With: __
Product Line: __
Quantity: 100 μL
Description: __
Label or Dye: __
Product Type: Antibody
For Use With (Application): __
Content And Storage:
-20°C
For Use With:
__
Product Line:
__
Quantity:
100 μL
Description:
__
Label or Dye:
__
Product Type:
Antibody
For Use With (Application):
__
Content And Storage: Software CD, License Key
For Use With: __
Product Line: __
Quantity: 1 License
Description: __
Label or Dye: __
Product Type: Software
For Use With (Application): Real Time PCR (qPCR)
Content And Storage:
Software CD, License Key
For Use With:
__
Product Line:
__
Quantity:
1 License
Description:
__
Label or Dye:
__
Product Type:
Software
For Use With (Application):
Real Time PCR (qPCR)