44-603-64
Applied Biosystems™ resDNASEQ™ Quantitative NS0 DNA Kits
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 44-603-64-Each-of-1 | In Stock | ₹ 3,43,095.60 |
44-603-64 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,43,095.60
GST (18%): ₹ 61,757.208
Total Price: ₹ 4,04,852.808
Content And Storage
resDNASEQ NS0 DNA Control• NS0 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ NS0 DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• NS0 DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format
Kit
Host Cell
NS0
Product Line
resDNASEQ™
Includes
Assay Kit and Sample Preparation
Detection Method
Primer-probe
PCR Method
qPCR
Label or Dye
FAM
No. of Reactions
100 Reactions
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Description
- The resDNASEQ™ Quantitative NS0 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines
- Reliable and rapid, the resDNASEQ™ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of NS0 cell DNA
- This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples to final product, whether the sample contains high molecular weight or sheared DNA
- Features of the resDNASEQ Quantitative NS0 DNA Kit include: • Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology • Consistent performance across the expected range of DNA fragment sizes • Integrated system includes sample preparation kit, master mix, TaqMan™ primer/probe mix, and NS0 genomic DNA standard This kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts
- The resDNASEQ™ Quantitative NS0 DNA Kit is designed to provide highly sensitive detection of NS0 DNA, allowing you to use small sample volumes to generate accurate results
- The broad linear range of TaqMan™ real-time qPCR technology allows you to test samples with variable levels of NS0 DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts
- Easy to use, results in under 4 hours The easy workflow of the resDNASEQ™ Quantitative NS0 DNA Kit consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform in under 4 hours
- Start by extracting DNA from your in-process purification and drug substance samples
- Then perform qPCR analysis to compare the amount of NS0 DNA in your test samples to a standard curve generated with known amounts of purified NS0 genomic DNA standard
- The resDNASEQ™ system includes almost everything you need to carry out these steps, from sample preparation kit to master mix to TaqMan™ primer/probe mix to NS0 genomic DNA standard, as well as step-by-step instructions
- Specific to NS0 DNA, no cross-reactivity with unrelated DNA The target of the assay is highly specific—it only detects a NS0-specific region of a multicopy genetic element
- We selected this region using extensive bioinformatic analysis of multiple related and unrelated species
- Consistent performance even with fragmented DNA For accurate quantitation of residual NS0 DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample
- With the resDNASEQ™ system you get consistent assay performance across a range of DNA fragment sizes.
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Content And Storage: resDNASEQ NS0 DNA Control• NS0 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ NS0 DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• NS0 DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format: Kit
Host Cell: NS0
Product Line: resDNASEQ™
Includes: Assay Kit and Sample Preparation
Detection Method: Primer-probe
PCR Method: qPCR
Label or Dye: FAM
No. of Reactions: 100 Reactions
Content And Storage:
resDNASEQ NS0 DNA Control• NS0 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ NS0 DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• NS0 DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format:
Kit
Host Cell:
NS0
Product Line:
resDNASEQ™
Includes:
Assay Kit and Sample Preparation
Detection Method:
Primer-probe
PCR Method:
qPCR
Label or Dye:
FAM
No. of Reactions:
100 Reactions
Content And Storage: -20°C
Format: __
Host Cell: __
Product Line: __
Includes: __
Detection Method: __
PCR Method: __
Label or Dye: __
No. of Reactions: __
Content And Storage:
-20°C
Format:
__
Host Cell:
__
Product Line:
__
Includes:
__
Detection Method:
__
PCR Method:
__
Label or Dye:
__
No. of Reactions:
__
Content And Storage: • Power SYBR Green PCR Master Mix (2X), store at -15°C to -25°C on receipt, 2°C to 8°C after first use, protected from light• Mycoplasma Real-Time PCR Primer Mix (10X), -15°C to -25°C on receipt, 2°C to 8°C after first use• Discriminatory Positive/Extraction Control, -15°C to -25°C• Negative control, -15°C to -25°C on receipt, 2°C to 8°C after first use
Format: __
Host Cell: __
Product Line: MycoSEQ™
Includes: __
Detection Method: __
PCR Method: __
Label or Dye: SYBR Green
No. of Reactions: __
Content And Storage:
• Power SYBR Green PCR Master Mix (2X), store at -15°C to -25°C on receipt, 2°C to 8°C after first use, protected from light• Mycoplasma Real-Time PCR Primer Mix (10X), -15°C to -25°C on receipt, 2°C to 8°C after first use• Discriminatory Positive/Extraction Control, -15°C to -25°C• Negative control, -15°C to -25°C on receipt, 2°C to 8°C after first use
Format:
__
Host Cell:
__
Product Line:
MycoSEQ™
Includes:
__
Detection Method:
__
PCR Method:
__
Label or Dye:
SYBR Green
No. of Reactions:
__
Content And Storage: MycoSEQ Mycoplasma Detection Kit• Power SYBR Green PCR Master Mix (2X), store at -15°C to -25°C on receipt, 2°C to 8°C after first use, protected from light• Mycoplasma Real-Time PCR Primer Mix (10X), -15°C to -25°C on receipt, 2°C to 8°C after first use• Discriminatory Positive/Extraction Control, -15°C to -25°C• Negative control, -15°C to -25°C on receipt, 2°C to 8°C after first usePrepSEQ 1-2-3 Mycoplasma Nucleic Acid Sample Extraction Kit• Cell Fractionation Buffer, store at 2°C to 8°C• RNase Cocktail Enzyme Mix, -15°C to -25°C• Lysis Buffer, 18°C to 25°C• Wash Buffer Concentrate, 18°C to 25°C• Elution Buffer, 18°C to 25°C• Proteinase K Buffer, 18°C to 25°C• Magnetic Particles, 18°C to 25°C• Proteinase K, -15°C to -25°C
Format: __
Host Cell: __
Product Line: MycoSEQ™
Includes: __
Detection Method: __
PCR Method: __
Label or Dye: SYBR Green
No. of Reactions: __
Content And Storage:
MycoSEQ Mycoplasma Detection Kit• Power SYBR Green PCR Master Mix (2X), store at -15°C to -25°C on receipt, 2°C to 8°C after first use, protected from light• Mycoplasma Real-Time PCR Primer Mix (10X), -15°C to -25°C on receipt, 2°C to 8°C after first use• Discriminatory Positive/Extraction Control, -15°C to -25°C• Negative control, -15°C to -25°C on receipt, 2°C to 8°C after first usePrepSEQ 1-2-3 Mycoplasma Nucleic Acid Sample Extraction Kit• Cell Fractionation Buffer, store at 2°C to 8°C• RNase Cocktail Enzyme Mix, -15°C to -25°C• Lysis Buffer, 18°C to 25°C• Wash Buffer Concentrate, 18°C to 25°C• Elution Buffer, 18°C to 25°C• Proteinase K Buffer, 18°C to 25°C• Magnetic Particles, 18°C to 25°C• Proteinase K, -15°C to -25°C
Format:
__
Host Cell:
__
Product Line:
MycoSEQ™
Includes:
__
Detection Method:
__
PCR Method:
__
Label or Dye:
SYBR Green
No. of Reactions:
__