Applied Biosystems™ resDNASEQ™ Quantitative HEK293 DNA Kits
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A46565-Each-of-1 | In Stock | ₹ 3,31,972.80 |
A46565 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,31,972.80
GST (18%): ₹ 59,755.104
Total Price: ₹ 3,91,727.904
Content And Storage
resDNASEQ HEK293 DNA Control• HEK293 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ HEK293 DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• HEK293 DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format
Kit
Host Cell
HEK
Product Line
resDNASEQ™
Includes
Assay Kit and Sample Preparation
Detection Method
Primer-probe
PCR Method
qPCR
Label or Dye
FAM
No. of Reactions
100 Reactions
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Description
- The resDNASEQ Quantitative HEK293 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host-cell DNA from HEK293 cell lines used in the development of gene therapy, cell-based vaccines, and similar biotherapeutics
- Reliable and rapid, the resDNASEQ system enables sensitive and specific quantitation of HEK293 cell DNA
- This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples with different sample matrices to purified final product
- Features of the resDNASEQ Quantitative HEK293 DNA Kit include: • Highly sensitive quantitation using proven TaqMan real-time qPCR technology • Consistent performance across the expected range of DNA fragment sizes • Includes master mix, TaqMan primer/probe mix, and HEK293 genomic DNA standard This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts
- Integrated workflow solution The resDNASEQ Quantitative HEK293 DNA Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing
- The kit is compatible with the PrepSEQ Residual DNA Sample Preparation Kit, which offers both a manual and automated sample preparation workflow
- The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the Applied Biosystems QuantStudio 5 Real-Time PCR System
- Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance
- Easy to use With the resDNASEQ Quantitative HEK293 DNA Kit, you perform qPCR analysis to compare the amount of HEK293 DNA in your test samples to a standard curve generated with known amounts of purified HEK293 DNA standard
- The resDNASEQ kit includes almost everything you need to carry out these steps, from master mix to TaqMan primer/probe mix to HEK293 genomic DNA standard, as well as step-by-step instructions
- Consistent performance For accurate quantitation of residual HEK293 DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample
- With the resDNASEQ system you get consistent assay performance across a range of DNA fragment sizes
- *Due to the high degree of similarity between genomes, DNA from closely-related primate species, such as monkey (Vero), chimpanzee, and orangutan, are expected be detected by the resDNASEQ HEK293 Quantitation Kit.
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Content And Storage: resDNASEQ HEK293 DNA Control• HEK293 DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ HEK293 DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• HEK293 DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format: Kit
Host Cell: HEK
Product Line: resDNASEQ™
Includes: Assay Kit and Sample Preparation
Detection Method: Primer-probe
PCR Method: qPCR
Label or Dye: FAM
No. of Reactions: 100 Reactions
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