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A50460

Applied Biosystems™ resDNASEQ™ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kits

Manufacturer: Fischer Scientific

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Pack Size	SKU	Availability	Price
Each of 1	A50460-Each-of-1	In Stock	₹ 3,66,196.80

A50460 - Each of 1

₹ 3,66,196.80

In Stock

Quantity

Base Price: ₹ 3,66,196.80

GST (18%): ₹ 65,915.424

Total Price: ₹ 4,32,112.224

Content And Storage

resDNASEQ KanR Control• KanR DNA Control, 40 μL, 3.0 x 107copies/μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ KanR DNA Real-Time PCR Reagents• Environmental Master Mix v3 (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• KanR DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.

Format

Kit

Label or Dye

FAM

Target

Kanamycin Resistant Plasmids

Includes

Assay Kit and Sample Preparation

Detection Method

Primer-probe

PCR Method

qPCR

Product Line

resDNASEQ™

No. of Reactions

100 Reactions

Related Products

Description

The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit is a quantitative PCR (qPCR)-based system for the detection of residual kanamycin-resistant plasmids used in the development of gene therapies, cell-based vaccines, and similar biotherapeutics
Reliable and rapid, the resDNASEQ system enables sensitive and specific quantitation of kanamycin-resistant plasmid DNA
This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples with different sample matrices to bulk drug substance
Features of the resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit include: • Designed to quantitate residual plasmid DNA with any common alleles of kanamycin resistance gene • Highly sensitive quantitation through innovative DNA-free master mix • Highly sensitive quantitation using proven TaqMan real-time PCR technology • Includes master mix, TaqMan primer/probe mix, kanamycin-resistant plasmid DNA standard, and sample preparation reagents This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts
Integrated workflow solution The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit with PrepSEQ Residual DNA Sample Preparation Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing
The included PrepSEQ Residual DNA Sample Preparation Kit offers both a manual and automated sample preparation workflow
The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the QuantStudio 5 Real-Time PCR System
Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance
Easy to use With the resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit, you perform qPCR analysis to compare the amount of kanamycin-resistant plasmid DNA in your test samples to standard curves generated with known amounts of kanamycin-resistant plasmid DNA standard
The resDNASEQ kit includes the components you need to carry out these steps, from master mix to TaqMan primer/probe mix to DNA standard, as well as step-by-step instructions.

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Fischer Scientific

A50460

Content And Storage:

resDNASEQ KanR Control• KanR DNA Control, 40 μL, 3.0 x 107copies/μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ KanR DNA Real-Time PCR Reagents• Environmental Master Mix v3 (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• KanR DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.

Format:

Kit

Label or Dye:

FAM

Target:

Kanamycin Resistant Plasmids

Includes:

Assay Kit and Sample Preparation

Detection Method:

Primer-probe

PCR Method:

qPCR

Product Line:

resDNASEQ™

No. of Reactions:

100 Reactions