Applied Biosystems™ resDNASEQ™ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kits

Description

• The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit is a quantitative PCR (qPCR)-based system for the detection of residual kanamycin-resistant plasmids used in the development of gene therapies, cell-based vaccines, and similar biotherapeutics
• Reliable and rapid, the resDNASEQ system enables sensitive and specific quantitation of kanamycin-resistant plasmid DNA
• This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples with different sample matrices to bulk drug substance
• Features of the resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit include: • Designed to quantitate residual plasmid DNA with any common alleles of kanamycin resistance gene • Highly sensitive quantitation through innovative DNA-free master mix • Highly sensitive quantitation using proven TaqMan real-time PCR technology • Includes master mix, TaqMan primer/probe mix, kanamycin-resistant plasmid DNA standard, and sample preparation reagents This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts
• Integrated workflow solution The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit with PrepSEQ Residual DNA Sample Preparation Kit is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing
• The included PrepSEQ Residual DNA Sample Preparation Kit offers both a manual and automated sample preparation workflow
• The resDNASEQ kit has been validated on the Applied Biosystems 7500 Fast Real-Time PCR System and the QuantStudio 5 Real-Time PCR System
• Data analysis is streamlined using AccuSEQ Real-Time PCR Detection Software that provides accurate quantitation and security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance
• Easy to use With the resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit, you perform qPCR analysis to compare the amount of kanamycin-resistant plasmid DNA in your test samples to standard curves generated with known amounts of kanamycin-resistant plasmid DNA standard
• The resDNASEQ kit includes the components you need to carry out these steps, from master mix to TaqMan primer/probe mix to DNA standard, as well as step-by-step instructions.