A4402085
Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kits
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A4402085-Each-of-1 | In Stock | ₹ 3,06,304.80 |
A4402085 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,06,304.80
GST (18%): ₹ 55,134.864
Total Price: ₹ 3,61,439.664
Content And Storage
resDNASEQ CHO DNA Control• CHO DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ CHO DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• CHO DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format
Kit
Host Cell
CHO
Product Line
resDNASEQ™
No. of Reactions
100 Reactions
Detection Method
Primer-probe
PCR Method
qPCR
Label or Dye
FAM
Shipping Condition
Dry Ice
Includes
Assay Kit only
Related Products
Description
- This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, enables you to detect sub-picogram amounts of residual DNA from the Chinese hamster ovary (CHO) cell line in under 5 hours
- The system overcomes the limitations of traditional methods by combining high-recovery PrepSEQ™ sample preparation and TaqMan™-based quantitation of residual DNA
- Real-time quantitative PCR kit for highly sensitive residual CHO DNA quantitation • Easy to use, results in under 5 hours • Specific to CHO DNA, no cross-reactivity with unrelated DNA • Part of an integrated system that includes sample preparation, TaqMan™ assay and master mix, standard DNA, instrument, and software • Protocol available for automated sample preparation • Flexible sample throughput allows you to perform rigorous DNA clearance studies This kit is available with or without sample preparation reagents in the form of a PrepSEQ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts
- Real-Time PCR for Highly Sensitive Quantitation The resDNASEQ™ Quantitative CHO DNA System provides highly sensitive detection of CHO DNA, allowing you to use small sample volumes to generate accurate results
- The broad linear range of TaqMan™ technology allows you to test samples with variable levels of CHO DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts
- Figure 1 demonstrates the range and sensitivity of the assay
- Linearity is demonstrated by analysis of CHO cell standard DNA ranging from 0.3 ng to 3 fg
- Easy to Use, Results in Under 5 Hours First, you extract DNA from your in-process purification and drug substance samples
- Then you perform qPCR to compare DNA amounts in your test samples to a standard curve generated with known amounts of purified CHO cell standard DNA
- The easy workflow of the resDNASEQ™ Quantitative CHO DNA System consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform in under 5 hours
- Specific to CHO DNA, No Cross-Reactivity With Unrelated DNA The target of the assay is highly specific, so that it only detects a hamster-specific region of a multicopy genetic element
- We selected this region using extensive bioinformatic analysis of multiple related and unrelated species
- Testing of assay performance confirmed that the assay is specific to hamster DNA and is unaffected by the presence of as much as 100 ng of unrelated DNA in a test sample
- Consistent Performance Even With Fragmented DNA For accurate quantitation of residual CHO DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample
- To test the effect of DNA fragment size on assay performance, we fragmented high molecular weight CHO genomic DNA into low molecular weight DNA by sonication
- Figure 2 demonstrates that the threshold cycle (Ct) values for the reactions with the sonicated low molecular weight DNA were comparable to those of the undigested high molecular weight DNA
- These results demonstrate that consistent performance was obtained with the kit irrespective of DNA molecular weight.
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Content And Storage: resDNASEQ CHO DNA Control• CHO DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ CHO DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• CHO DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format: Kit
Host Cell: CHO
Product Line: resDNASEQ™
No. of Reactions: 100 Reactions
Detection Method: Primer-probe
PCR Method: qPCR
Label or Dye: FAM
Shipping Condition: Dry Ice
Includes: Assay Kit only
Content And Storage:
resDNASEQ CHO DNA Control• CHO DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ CHO DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• CHO DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format:
Kit
Host Cell:
CHO
Product Line:
resDNASEQ™
No. of Reactions:
100 Reactions
Detection Method:
Primer-probe
PCR Method:
qPCR
Label or Dye:
FAM
Shipping Condition:
Dry Ice
Includes:
Assay Kit only
Content And Storage: __
Format: __
Host Cell: __
Product Line: __
No. of Reactions: __
Detection Method: __
PCR Method: __
Label or Dye: __
Shipping Condition: __
Includes: __
Content And Storage:
__
Format:
__
Host Cell:
__
Product Line:
__
No. of Reactions:
__
Detection Method:
__
PCR Method:
__
Label or Dye:
__
Shipping Condition:
__
Includes:
__
Content And Storage: resDNASEQ CHO DNA Control• CHO DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ CHO DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• CHO DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format: Kit
Host Cell: CHO
Product Line: resDNASEQ™
No. of Reactions: 100 Reactions
Detection Method: Primer-probe
PCR Method: qPCR
Label or Dye: FAM
Shipping Condition: __
Includes: Assay Kit and Sample Preparation
Content And Storage:
resDNASEQ CHO DNA Control• CHO DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ CHO DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• CHO DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format:
Kit
Host Cell:
CHO
Product Line:
resDNASEQ™
No. of Reactions:
100 Reactions
Detection Method:
Primer-probe
PCR Method:
qPCR
Label or Dye:
FAM
Shipping Condition:
__
Includes:
Assay Kit and Sample Preparation
Content And Storage: __
Format: __
Host Cell: __
Product Line: __
No. of Reactions: __
Detection Method: __
PCR Method: __
Label or Dye: __
Shipping Condition: __
Includes: __
Content And Storage:
__
Format:
__
Host Cell:
__
Product Line:
__
No. of Reactions:
__
Detection Method:
__
PCR Method:
__
Label or Dye:
__
Shipping Condition:
__
Includes:
__