Applied Biosystems™ resDNASEQ™ Quantitative NS0 DNA Kits

Description

• The resDNASEQ™ Quantitative NS0 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines
• Reliable and rapid, the resDNASEQ™ system enables sensitive (as low as 1.5 pg DNA/mL test sample) and specific quantitation of NS0 cell DNA
• This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types, from in-process samples to final product, whether the sample contains high molecular weight or sheared DNA
• Features of the resDNASEQ Quantitative NS0 DNA Kit include: • Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology • Consistent performance across the expected range of DNA fragment sizes • Integrated system includes sample preparation kit, master mix, TaqMan™ primer/probe mix, and NS0 genomic DNA standard This kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts
• The resDNASEQ™ Quantitative NS0 DNA Kit is designed to provide highly sensitive detection of NS0 DNA, allowing you to use small sample volumes to generate accurate results
• The broad linear range of TaqMan™ real-time qPCR technology allows you to test samples with variable levels of NS0 DNA in the same assay, such as in-process samples with higher amounts of DNA or bulk drug substance with very low amounts
• Easy to use, results in under 4 hours The easy workflow of the resDNASEQ™ Quantitative NS0 DNA Kit consists of sample preparation, assay setup, and instrument run and data analysis—all of which you can perform in under 4 hours
• Start by extracting DNA from your in-process purification and drug substance samples
• Then perform qPCR analysis to compare the amount of NS0 DNA in your test samples to a standard curve generated with known amounts of purified NS0 genomic DNA standard
• The resDNASEQ™ system includes almost everything you need to carry out these steps, from sample preparation kit to master mix to TaqMan™ primer/probe mix to NS0 genomic DNA standard, as well as step-by-step instructions
• Specific to NS0 DNA, no cross-reactivity with unrelated DNA The target of the assay is highly specific—it only detects a NS0-specific region of a multicopy genetic element
• We selected this region using extensive bioinformatic analysis of multiple related and unrelated species
• Consistent performance even with fragmented DNA For accurate quantitation of residual NS0 DNA, assay results must be unaffected by the size of the DNA molecules present in the test sample
• With the resDNASEQ™ system you get consistent assay performance across a range of DNA fragment sizes.