A4458435
Applied Biosystems™ resDNASEQ™ QuantitativeE. coliDNA Kits
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A4458435-Each-of-1 | In Stock | ₹ 3,06,304.80 |
A4458435 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 3,06,304.80
GST (18%): ₹ 55,134.864
Total Price: ₹ 3,61,439.664
Content And Storage
resDNASEQ E.coli DNA Control• E.coli DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ E.coli DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• E.coli DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format
Kit
Host Cell
E.coli
Product Line
resDNASEQ™
No. of Reactions
100 Reactions
Detection Method
Primer-probe
PCR Method
qPCR
Label or Dye
FAM
Shipping Condition
Dry Ice
Includes
Assay Kit only
Related Products
Description
- This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E
- coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E
- coli expression systems, typically in less than 4 hours
- The kit is part of an integrated system that includes sample preparation, TaqMan™ Assay and master mix, standard DNA, instrument, and software
- The system overcomes the limitations of traditional methods by combining high-recovery PrepSEQ™ sample preparation and TaqMan™ Assay-based quantitation of residual DNA
- Features of the resDNASEQ Quantitative E
- coli DNA Kit include: • Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology • Manual and automated sample preparation, optimized for quantitative recovery from complex sample matrices (Table 1) • Consistent performance across the expected range of DNA fragment sizes • Integrated system from sample to results with sample preparation, master mix, TaqMan™ primer/probe mix, and genomic DNA standard This kit is available with or without sample preparation reagents in the form of a PrepSEQ™ Residual DNA Sample Preparation Kit, which is optimized for highly efficient residual DNA recovery from complex mixtures of proteins, buffers, and salts
- Rapid, Sensitive Detection of E.Coli DNA The resDNASEQ™ E
- coli Residual DNA Quantitation System is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from E
- coli , an expression system commonly used for the production of recombinant proteins
- Reliable and rapid, the system enables sensitive (LOQ = 15 pg DNA/mL test sample) and specific quantitation of E
- coli DNA, typically in less than 4 hours
- This performance helps ensure a high degree of confidence in quantitation data obtained from a broad range of sample types—from in-process samples to bulk drug substances—whether a sample contains high molecular weight or sheared DNA
- Table 1
- DNA recovery using manual PrepSEQ™ sample preparation protocol
- Assay performance data using 10 pg E
- coli genomic DNA spike per sample, 3 analysts, and 9 test samples
- 50 mg/mL protein sample Mean Recovery Mean %CV E
- coli 83% 5.04%
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Content And Storage: resDNASEQ E.coli DNA Control• E.coli DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ E.coli DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• E.coli DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format: Kit
Host Cell: E.coli
Product Line: resDNASEQ™
No. of Reactions: 100 Reactions
Detection Method: Primer-probe
PCR Method: qPCR
Label or Dye: FAM
Shipping Condition: Dry Ice
Includes: Assay Kit only
Content And Storage:
resDNASEQ E.coli DNA Control• E.coli DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ E.coli DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• E.coli DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first use
Format:
Kit
Host Cell:
E.coli
Product Line:
resDNASEQ™
No. of Reactions:
100 Reactions
Detection Method:
Primer-probe
PCR Method:
qPCR
Label or Dye:
FAM
Shipping Condition:
Dry Ice
Includes:
Assay Kit only
Content And Storage: resDNASEQ E.coli DNA Control• E.coli DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ E.coli DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• E.coli DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format: Kit
Host Cell: E.coli
Product Line: resDNASEQ™
No. of Reactions: 100 Reactions
Detection Method: Primer-probe
PCR Method: qPCR
Label or Dye: FAM
Shipping Condition: __
Includes: Assay Kit and Sample Preparation
Content And Storage:
resDNASEQ E.coli DNA Control• E.coli DNA Control, 30 ng/μL, 40 μL, store at –15 to –25°C• DNA Dilution Buffer (DDB), 7 mL, store at –15 to –25°C before first use and 2−8°C after first useresDNASEQ E.coli DNA Real-Time PCR Reagents• Environmental Master Mix (2X), 2 x 0.75 mL, store at –15 to –25°C before first use and 2−8°C after first use, protect from light• E.coli DNA Assay Mix (10X), 300 μL, store at –15 to –25°C, protect from light• Negative Control (water), 1.0 mL, store at –15 to –25°C before first use and 2−8°C after first usePrepSEQ Nucleic Acid Extraction Kit• Lysis Buffer (2 bottles, 50 mL/bottle)• Binding Solution/Isopropanol (1 empty bottle)• Wash Buffer Concentrate (2 bottles, 26 mL/bottle)• Elution Buffer (1 bottle, 25 mL)• Proteinase K Buffer (1 bottle, 50 mL)• Magnetic Particles (2 tubes, 1.5 mL/tube)• Proteinase K (20 mg/mL) (1 tube, 1.25 mL), store at or below –20°C• PK Buffer II (1 bottle, 11 mL)Store all at room temperature unless otherwise indicated.PrepSEQ Residual DNA Sample Preparation Kit• Proteinase K (20 mg/mL), 1 tube, 1.25 mL• Yeast tRNA (10 mg/mL), 1 tube, 0.5 mL• Glycogen (5 mg/mL), 2 tubes, 1.0 mL/tubeStore all at or below –20°C.
Format:
Kit
Host Cell:
E.coli
Product Line:
resDNASEQ™
No. of Reactions:
100 Reactions
Detection Method:
Primer-probe
PCR Method:
qPCR
Label or Dye:
FAM
Shipping Condition:
__
Includes:
Assay Kit and Sample Preparation
Content And Storage: __
Format: __
Host Cell: __
Product Line: __
No. of Reactions: __
Detection Method: __
PCR Method: __
Label or Dye: __
Shipping Condition: __
Includes: __
Content And Storage:
__
Format:
__
Host Cell:
__
Product Line:
__
No. of Reactions:
__
Detection Method:
__
PCR Method:
__
Label or Dye:
__
Shipping Condition:
__
Includes:
__
Content And Storage: • 2X Platinum Direct PCR Universal Master Mix (1 mL)• Lysis Buffer (5 mL)• Proteinase K (150 μL)• Platinum GC Enhancer (1.25 mL)• Nuclease-free water (1.25 mL)Store at –20°C.
Format: __
Host Cell: __
Product Line: __
No. of Reactions: 100 Reactions
Detection Method: __
PCR Method: __
Label or Dye: __
Shipping Condition: Dry Ice
Includes: __
Content And Storage:
• 2X Platinum Direct PCR Universal Master Mix (1 mL)• Lysis Buffer (5 mL)• Proteinase K (150 μL)• Platinum GC Enhancer (1.25 mL)• Nuclease-free water (1.25 mL)Store at –20°C.
Format:
__
Host Cell:
__
Product Line:
__
No. of Reactions:
100 Reactions
Detection Method:
__
PCR Method:
__
Label or Dye:
__
Shipping Condition:
Dry Ice
Includes:
__