Thermo Scientific™ Phusion High-Fidelity PCR Kit

Description

• Thermo Scientific Phusion High-Fidelity DNA Polymerase sets a high standard for high performance PCR
• Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu , Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity
• Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases
• The Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons
• The DNA template amount is sufficient for performing 20 control reactions in 50 μL volume or 50 control reactions in 20 μL volume
• Highlights High fidelity (52X Taq ) Fast PCR due to short extension times (15–30 s/kb) Robust performance, minimal optimization needed Increased product yields with minimal enzyme amounts Applications High-fidelity PCR High throughput Amplification of difficult (GC-rich) templates Template generation for sequencing Multiplex PCR Long-range PCR Cloning & mutagenesis Microarray Notes Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).