F553L
Thermo Scientific™ Phusion High-Fidelity PCR Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F553L-Each-of-1 | In Stock | ₹ 33,026.16 |
F553L - Each of 1
In Stock
Quantity
1
Base Price: ₹ 33,026.16
GST (18%): ₹ 5,944.709
Total Price: ₹ 38,970.869
Content And Storage
• Phusion DNA Polymerase (2 U/μL)• 5X Phusion GC & HF Buffer• dNTP mix (10 mM each)• Control template (λ DNA) and DNA size standard• 1.3 kb & 10 kb primers (4 μM each)• 50 mM MgCl2solution and DMSOStore at –20°C.
Polymerase
Phusion High-Fidelity DNA Polymerase
Product Type
High-Fidelity PCR Kit
For Use With (Application)
Standard PCR, High-fidelity PCR
Hot Start
No
Overhang
Blunt
Size (Final Product)
20 kb or less
GC-Rich PCR Performance
High
Reaction Speed
Fast
Shipping Condition
Dry Ice
Fidelity (vs. Taq)
52X
No. of Reactions
200 Reactions
Reaction Format
Separate Components
Related Products
Description
- Thermo Scientific Phusion High-Fidelity DNA Polymerase sets a high standard for high performance PCR
- Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu , Phusion High-Fidelity DNA Polymerase is an excellent choice for cloning and other applications requiring high fidelity
- Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerases
- The Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control lambda DNA template and primers for 1.3 kb and 10 kb amplicons
- The DNA template amount is sufficient for performing 20 control reactions in 50 μL volume or 50 control reactions in 20 μL volume
- Highlights High fidelity (52X Taq ) Fast PCR due to short extension times (15–30 s/kb) Robust performance, minimal optimization needed Increased product yields with minimal enzyme amounts Applications High-fidelity PCR High throughput Amplification of difficult (GC-rich) templates Template generation for sequencing Multiplex PCR Long-range PCR Cloning & mutagenesis Microarray Notes Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
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Content And Storage: • Phusion DNA Polymerase (2 U/μL)• 5X Phusion GC & HF Buffer• dNTP mix (10 mM each)• Control template (λ DNA) and DNA size standard• 1.3 kb & 10 kb primers (4 μM each)• 50 mM MgCl2solution and DMSOStore at –20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Product Type: High-Fidelity PCR Kit
For Use With (Application): Standard PCR, High-fidelity PCR
Hot Start: No
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Fidelity (vs. Taq): 52X
No. of Reactions: 200 Reactions
Reaction Format: Separate Components
Content And Storage:
• Phusion DNA Polymerase (2 U/μL)• 5X Phusion GC & HF Buffer• dNTP mix (10 mM each)• Control template (λ DNA) and DNA size standard• 1.3 kb & 10 kb primers (4 μM each)• 50 mM MgCl2solution and DMSOStore at –20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Product Type:
High-Fidelity PCR Kit
For Use With (Application):
Standard PCR, High-fidelity PCR
Hot Start:
No
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Fidelity (vs. Taq):
52X
No. of Reactions:
200 Reactions
Reaction Format:
Separate Components
Content And Storage: • Phusion DNA Polymerase (2 U/μL)• 5X Phusion GC & HF Buffer• dNTP mix (10 mM each)• Control template (λ DNA) and DNA size standard• 1.3 kb & 10 kb primers (4 μM each)• 50 mM MgCl2solution and DMSOStore at –20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Product Type: High-Fidelity PCR Kit
For Use With (Application): Standard PCR, High-fidelity PCR
Hot Start: No
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Fidelity (vs. Taq): 52X
No. of Reactions: 50 Reactions
Reaction Format: Separate Components
Content And Storage:
• Phusion DNA Polymerase (2 U/μL)• 5X Phusion GC & HF Buffer• dNTP mix (10 mM each)• Control template (λ DNA) and DNA size standard• 1.3 kb & 10 kb primers (4 μM each)• 50 mM MgCl2solution and DMSOStore at –20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Product Type:
High-Fidelity PCR Kit
For Use With (Application):
Standard PCR, High-fidelity PCR
Hot Start:
No
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Fidelity (vs. Taq):
52X
No. of Reactions:
50 Reactions
Reaction Format:
Separate Components
Content And Storage: • Phusion U Hot Start DNA Polymerase, 2 U/μL• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase: Phusion U Hot Start DNA Polymerase
Product Type: Hot Start DNA Polymerase
For Use With (Application): Hot-start PCR, High-fidelity PCR
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Fidelity (vs. Taq): 25X
No. of Reactions: 500 Reactions
Reaction Format: Standalone
Content And Storage:
• Phusion U Hot Start DNA Polymerase, 2 U/μL• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase:
Phusion U Hot Start DNA Polymerase
Product Type:
Hot Start DNA Polymerase
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Fidelity (vs. Taq):
25X
No. of Reactions:
500 Reactions
Reaction Format:
Standalone
Content And Storage: • Phusion U Hot Start DNA Polymerase, 2 U/μL• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase: Phusion U Hot Start DNA Polymerase
Product Type: Hot Start DNA Polymerase
For Use With (Application): Hot-start PCR, High-fidelity PCR
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Fidelity (vs. Taq): 25X
No. of Reactions: 100 Reactions
Reaction Format: Standalone
Content And Storage:
• Phusion U Hot Start DNA Polymerase, 2 U/μL• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase:
Phusion U Hot Start DNA Polymerase
Product Type:
Hot Start DNA Polymerase
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Fidelity (vs. Taq):
25X
No. of Reactions:
100 Reactions
Reaction Format:
Standalone