Thermo Scientific™ Melon™ Gel Monoclonal IgG Purification Kit

Description

• Thermo Scientific™ Melon Gel Monoclonal IgG Purification Kit is a high-yield, mild purification system for mouse monoclonal antibodies from serum, ascites or hybridoma cell culture supernatant
• The Melon Gel Monoclonal IgG Purification Kit consists of a large package size of Melon Gel Resin and an optimized protocol for easy and mild purification of monoclonal IgG antibodies from hybridoma culture supernatant or ascites fluid
• Unlike typical bind-and-elute affinity methods, Melon Gel Purification is based on the binding of non-IgG proteins from a prepared supernatant or ascites sample, thereby allowing the pure IgG to be recovered in the flow-through (non-bound) fraction
• Antibodies are recovered ready for use in assays or subsequent conjugation and other modification steps
• There is no need to neutralize or desalt to mitigate the effects of harsh elution buffers
• Melon Gel Purification saves time compared to classical Protein A or Protein G methods without compromising the quality of the antibody
• Highlights: Fast – purify antibodies in half the time of required for Protein A or G Efficient – recovery and purity is often better than Protein A or G purification Robust – effective for subclasses of IgG that bind poorly to Protein A or G Gentle – antibodies retain activity because they are not exposed to denaturing conditions Reusable – support can be regenerated and used for up to 6 purifications Compatible – amine-free buffer does not require removal or neutralization Flexible – accommodates a broad range of sample volumes and processing vessels Includes: Melon Gel Resin, purification buffer and resin regenerant