Description

• PROTAC targeting mutant FKBP12F36V fusion proteins
• Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand
• FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques (via transgene expression or CRISPR-mediated locus-specific knock-in)
• Application of dTAG-13 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo
• dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation
• Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene
• dTAG-13 is a degrader targeting mutant FKBP12F36V fusion proteins
• Comprises a ligand selective for F36V single-point mutated FKBP12, a linker and a cereblon-binding ligand
• Application of dTAG-13 induces rapid, reversible and selective degradation of FKBP12F36V fusion proteins in vitro and in vivo
• dTAG is generalizable to a range of fusion proteins; useful as an alternative to genetic methods for target validation
• Negative control (Cat
• No
• 6916) also available
• FKBP12F36V can be expressed as a fusion with a target protein of interest using genome engineering techniques, via transgene expression or CRISPR-mediated locus-specific knock-in
• Custom knock-in cell lines for the dTAG and aTAG platforms are available from our sister brand R&D Systems
• Email TPD@bio-techne.com to enquire
• Plasmid vectors for the lentiviral expression and CRISPR-mediated knock-in of FKBP12F36V are available from Addgene.