69-451-3

MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 69-451-3-Each-of-1 In Stock ₹ 15,998.86

69-451-3 - Each of 1

₹ 15,998.86

In Stock

Quantity

1

Base Price: ₹ 15,998.86

GST (18%): ₹ 2,879.795

Total Price: ₹ 18,878.655

Efficiency

Guaranteed efficiency: >2 x 107cfu/μg

Content And Storage

≤-70°C

Quantity

0.4 mL

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Description

  • BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases
  • DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter
  • Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG
  • pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.

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Efficiency:
Guaranteed efficiency: >2 x 107cfu/μg

Content And Storage:
≤-70°C

Quantity:
0.4 mL

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Guaranteed efficiency: >5 x 106cfu/μg

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Efficiency:
Guaranteed efficiency: >5 x 106cfu/μg

Content And Storage:
≤-70°C

Quantity:
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