Molecular Probes™ Alexa Fluor™ 633 Hydrazide

Description

• Alexa Fluor™ 633 Hydrazide is useful as a cell tracer and as a reactive dye for labeling aldehydes or ketones in polysaccharides or glycoproteins
• Alexa Fluor™ 633 is a bright, far red fluorescent dye with excitation ideally suited to the 633 nm laser line
• Used for stable signal generation in imaging and flow cytometry, Alexa Fluor™ 633 dye is water soluble and pH-insensitive from pH 4 to pH 10
• To make a concentrated stock solution from 1–10 mM, dissolve the dye-hydrazide in either H 2 O or DMSO
• Store frozen and protected from light
• Detailed information about this AlexaFluor™ hydrazide: • Fluorophore label: Alexa Fluor™ 633 dye • Reactive group: hydrazide • Reactivity: Aldehydes or keytones in polysaccharides or glycoproteins • Ex/Em of the conjugate: 624/643 nm • Extinction coefficient: 110,000 cm-1M-1 • Spectrally similar dyes: Cy5 • Molecular weight: ∼1,150 Cell Tracking and Tracing Applications Alexa Fluor™ hydrazides and hydroxlamines are useful as low molecular weight, membrane-impermeant, aldehyde-fixable cell tracers, exhibiting brighter fluorescence and greater photostability than cell tracers derived from other spectrally similar fluorophores
• They are easily loaded into cells by microinjection, infusion from patch pipette, or uptake induced by our Influx™ Pinocytic Cell-Loading Reagent (Cat
• No
• I14402)
• Glycoprotein and Polysaccharide Labeling Applications The Alexa Fluor™ hydrazides and hydroxlamines are reactive molecules that can be used to add a fluorescent label to biomolecules containing aldehydes or ketones
• Aldehydes and ketones can be introduced into polysaccharides and glycoproteins by periodate-mediated oxidation of vicinal diols
• Galactose oxidase can also be used to oxidize terminal galactose residues of glycoproteins to aldehydes
• Hydrazide vs Hydroxylamine Hydrazine derivatives react with ketones and aldehydes to yield relatively stable hydrazones
• Hydroxylamine derivatives (aminooxy compounds) react with aldehydes and ketones to yield oximes
• Oximes are superior to hydrazones with respect to hydrolytic stability
• Both hydrazones and oximes can be reduced with sodium borohydride (NaBH 4 ) to further increase the stability of the linkage
• Learn More About Protein and Antibody Labeling We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup
• See our Antibody Labeling kits or use our Labeling Chemistry Selection Tool for other choices
• To learn more about our labeling kits, read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in The Molecular Probes™ Handbook
• We’ll Make a Custom Conjugate for You If you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you
• Our custom conjugation service is efficient and confidential, and we stand by the quality of our work
• We are ISO 13485:2000 certified
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