Thermo Scientific™ Pierce™ Alkylating Reagents

Description

• Thermo Scientific Pierce Iodoacetamide or Chloroacetamide are provided in a no-weigh format, where small amounts of reagent are pre-weighed and packaged for single use
• This single-use format prevents the contamination and loss of reagent reactivity over time associated with repetitive opening and closing of a single vial
• The format enables use of a fresh vial of reagent each time, and eliminates the hassle of weighing out small amounts of reagent
• Alkylating reagents iodoacetamide and chloracetamide are sulfhydryl-reactive alkylating reagents used to block reduced cysteine residues for protein characterization and peptide mapping
• Alkylation with iodoacetamide or chloracetamide after cysteine reduction results in the covalent addition of a carbamidomethyl group (57.07 Da) and prevents the formation of disulfide bonds
• Reducing agents added after alkylation will react with excess iodoacetamide.