Invitrogen™ ZOOM™ Urea

Description

• The ZOOM™ IEF Fractionator offers a fast, reliable method to reduce sample complexity, enrich low abundance proteins, and increase the dynamic range of detection
• Solution phase isoelectric focusing with the ZOOM™ IEF Fractionator provides reproducible separations in three hours
• Fractionated samples are ready for further analysis by two dimensional gel electrophoresis (2DE), one dimensional gel electrophoresis (1DE), or two dimensional liquid chromatography/mass spectrometry (2D LC/MS)
• The ZOOM™ IEF Fractionator System (Figure 1) consists of the following components: ZOOM™ IEF Fractionator is built for safety, reliability, and durability
• It includes easy-to-assemble sample chambers, in-built buffer chambers, buffer trough, removable electrode assembly, and safety lid
• ZOOM™ Disks are pre-cast polyacrylamide gels that eliminate the need for manual preparation and minimize any chance of cross contamination
• These immobilized buffered disks are pre-labeled, disposable, and designed for single use, ensuring consistent and reproducible fractionation
• They resolve samples into as many as 7 fractions (using 8 disks of specific pH) to six fractions (using six disks of specific pH) from pH 3-12
• Simply place each disk between the sample chambers in the ZOOM™ IEF Fractionator to allow separation in a specific pH range
• ZOOM™ Reagents are proteomic grade reagents, including ZOOM™ 2D Protein Solubilizers urea, thiourea, CHAPS, and ampholytes, that ensure optimal conditions for sample preparation
• Basic Protein Kit which includes ZOOM™ Discs and ZOOM Strips for IEF of basic proteins, focusing buffers, and solubilizer kits
• Sample Chamber and Fractionator Unit Set-up Procedure Figures 4-6 illustrate assembly of the sample chambers and fractionator unit
• The sample chamber and components fit together easily and offer a leak-proof seal
• Simply place disks between the sample chambers in the ZOOM™ IEF Fractionator to allow separation in a specific pH range
• For example, if you want to fractionate proteins between pH 4.6 and 5.4, flank a sample chamber with a ZOOM (r) Disk of pH 4.6 and one of pH 5.4
• Downstream Processing by 2D Gel Electrophoresis Following fractionation, the separated samples can be subjected to further analysis using narrow or broad range ZOOM™ Strips on the ZOOM™ IPGRunner™ System
• Follow with second dimension analysis using neutral pH NuPAGE™ ZOOM™ Gels.