BDB611473
BD Mouse Macrophage plus IFNγ/LPS Lysate
Manufacturer: BD
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Tissue
Macrophage
Quantity
500 μg
Host Species
Mouse
Content And Storage
-20°C
Preparation Method
Mouse macrophages (RAW 264.7 cells) were grown to 70% confluencey, then stimulated with 10ng/mL IFNgamma and 1μg/mL LPS for 12 Hours, western blotting of mouse macrophages stimulated with IFNgamma and LPS
Storage Buffer
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Concentration
1 mg/mL
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Description
- Source is rich in endogenously expressed proteins Provided in denatured format allowing for immediate loading onto SDS-PAGE gel Storage buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol) Routinely tested western blot
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Tissue: Macrophage
Quantity: 500 μg
Host Species: Mouse
Content And Storage: -20°C
Preparation Method: Mouse macrophages (RAW 264.7 cells) were grown to 70% confluencey, then stimulated with 10ng/mL IFNgamma and 1μg/mL LPS for 12 Hours, western blotting of mouse macrophages stimulated with IFNgamma and LPS
Storage Buffer: 1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Concentration: 1 mg/mL
Tissue:
Macrophage
Quantity:
500 μg
Host Species:
Mouse
Content And Storage:
-20°C
Preparation Method:
Mouse macrophages (RAW 264.7 cells) were grown to 70% confluencey, then stimulated with 10ng/mL IFNgamma and 1μg/mL LPS for 12 Hours, western blotting of mouse macrophages stimulated with IFNgamma and LPS
Storage Buffer:
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Concentration:
1 mg/mL
Tissue: Macrophage
Quantity: 500 μg
Host Species: Mouse
Content And Storage: -20°C
Preparation Method: __
Storage Buffer: 1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Concentration: 1 mg/mL
Tissue:
Macrophage
Quantity:
500 μg
Host Species:
Mouse
Content And Storage:
-20°C
Preparation Method:
__
Storage Buffer:
1mg/mL in SDS-PAGE Buffer: 62mM Tris pH 6.8, 2% SDS, 0.9% beta-mercaptoethanol, 0.003% Bromophenol Blue, 5% Glycerol
Concentration:
1 mg/mL
Tissue: __
Quantity: 50 μg
Host Species: Mouse
Content And Storage: Store undiluted at -20°C.
Preparation Method: __
Storage Buffer: __
Concentration: 250μg/mL
Tissue:
__
Quantity:
50 μg
Host Species:
Mouse
Content And Storage:
Store undiluted at -20°C.
Preparation Method:
__
Storage Buffer:
__
Concentration:
250μg/mL
Tissue: __
Quantity: 150 μg
Host Species: Mouse
Content And Storage: Store undiluted at -20°C.
Preparation Method: __
Storage Buffer: __
Concentration: 250μg/mL
Tissue:
__
Quantity:
150 μg
Host Species:
Mouse
Content And Storage:
Store undiluted at -20°C.
Preparation Method:
__
Storage Buffer:
__
Concentration:
250μg/mL