Nedd4 Mouse anti-Mouse, Unlabeled, Clone: 15, BD

Description

• Regulated protein degradation involves conjugation of ubiquitin to proteins for degradation by the 26S proteasome
• Protein ubiquitination is the end product of a multienzyme cascade that involves sequential thiol-ester bond interactions between ubiquitin and enzymes termed E1-E3
• Neuronal precursor cell-expressed developmentally down-regulated 4 (Nedd4) is a multimodular ubiquitin protein ligase E3 that contains a Ca 2+ ;-lipid binding domain (CaLB), 3 WW domains, and a C-terminal ubiquitin protein ligase hect domain
• The CaLB is characteristic of C2 domain-containing proteins, such as PKC, phospholipase C, phospholipase A2, and synaptogamin
• Nedd4 is expressed differentially in the fetal and adult brain and its highest expression is in lung and kidney
• Although it localizes in the cytoplasm, increased intracellular Ca 2+ induces plasma membrane association of Nedd4
• In addition, increases in intracellular Na + leads to Nedd4 binding to epithelial Na + channels via its WW domains, which results in targeting of Na + channels for degradation
• Thus, the subcellular localization and protein interactions of Nedd4 are regulated in a Ca 2+ ;- and Na+-dependent manner
• Host Species: Mouse Clone: 15 Isotype: IgG1 Species Reactivity: Mouse Immunogen: Mouse Nedd4 aa
• 173-262 Formula Weight [Chemical]: 110kDa Immunofluorescence, Western Blotting