Itch Mouse, Unlabeled, Clone: 32, BD

Description

• Maintenance of cellular function requires timely and selective degradation of key regulatory proteins
• For example, progression of the mammalian cell cycle is regulated by phosphorylation/dephosphorylation and synthesis/degradation of many key proteins via the ubiquitin pathway
• Ubiquitin, a soluble protein of 76 amino acids, is enzymatically attached to an ε-NH2-Lys in a target protein
• Ubiquitin-conjugated proteins are recognized and degraded by the 26S proteasome
• Ubiquitination requires ubiquitin-activating enzyme E1, ubiquitin-conjugating enzymes E2, and ubiquitin ligases E3
• The direction of ubiquitin transfer is from E1 to E2 and from E2 to E3
• Itch, a novel E3 ubiquitin ligase, is absent in the Non-agouti-lethal 18H mice
• These mice develop immunological, inflammatory, epithelial, and hematopoietic diseases
• Itch contains four WW protein interaction domains, which bind to proline-rich sequences in a fashion similar to SH3 domains
• In addition, Itch contains a C-terminal Hect domain, which is conserved in the E3 family of ubiquitin ligases
• Thus, Itch is important in the ubiquitin-dependent protein degradation occurring in normal hematopoiesis and inflammation
• Host Species: Mouse Clone: 32 Isotype: IgG1 Species Reactivity: Rat Immunogen: Mouse Itch aa
• 114-220 Formula Weight [Chemical]: 113kDa Immunofluorescence, Western Blotting