50-257-6137
Invitrogen™ BACE1 (β-Secretase) FRET Assay Kit, red
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 50-257-6137-Each-of-1 | In Stock | ₹ 1,20,810.72 |
50-257-6137 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,20,810.72
GST (18%): ₹ 21,745.93
Total Price: ₹ 1,42,556.65
Content And Storage
Components of the BACE1 FRET Assay Kit are listed in Table 1. Sufficient reagent is supplied for 400 high-throughput kinetic or endpoint assays. The BACE1 Enzyme is also available separately. Store BACE1 Enzyme at -80°C. Store BACE1 Substrate and BACE1 Product Standard at -20°C. Avoid subjecting these components to repeated freeze-thaw cycles. The BACE1 Assay Buffer and BACE1 Stop Solution can be thawed upon receipt and stored at room temperature.
Product Type
FRET Assay Kit
Substrate Type
Beta-Secretase Substrate
Quantity
400 x 40 μL Assays
Target
Beta Secretase (BACE1)
Assay
β-Secretase Assay
Substrate Properties
Peptide-Based Substrate
For Use With (Application)
FRET
Shipping Condition
Dry Ice
Related Products
Description
- BACE1 (β-secretase) is a key enzyme involved in the production of Amyloid beta-peptides (Abeta) found in extracellular amyloid plaques of Alzheimers disease (AD)
- In some cases, early onset familial AD can be attributed to a Swedish mutation in the amyloid precursor protein (APP), dramatically enhancing the cleavage of this protein by BACE1
- This and other genetic and pathological evidence has led to therapeutic approaches focusing on the inhibition of BACE1 and other APP-cleaving enzymes, such as gamma-secretase
- The BACE1 fluorescence resonance energy transfer (FRET) Assay Kit provides a sensitive and efficient method for screening potential BACE1 inhibitors
- This kit uses purified baculovirus-expressed BACE1 and a new red FRET peptide substrate based on the Swedish mutant
- The BACE1 FRET Assay Kit offers: Red emission to reduce compound interference Excitation at 545 nm and read emission at 585 nm An easy-to-use, rapid, homogeneous assay that is performed in solution Versatile formatting for high-throughput screening Stable results—measurements can be made for up to 24 hours The principle of the BACE1 FRET assay is as follows: The peptide substrate is synthesized with two fluorophores, a fluorescent donor (a rhodamine (Rh) derivative), and a proprietary quenching acceptor
- The distance between these two groups has been selected so that upon light excitation, the donor (D) fluorescence energy is significantly quenched by the acceptor (A) through a quantum mechanical phenomenon known as resonance energy transfer (Figure 1)
- Upon cleavage by the protease, the fluorophore is separated from the quenching group, restoring the full fluorescence yield of the donor
- Thus, a weakly fluorescent peptide substrate becomes highly fluorescent upon enzymatic cleavage; the increase in fluorescence is linearly related to the rate of proteolysis
- Source of BACE1 Enzyme: A cDNA sequence encoding amino acids 1-460 of human BACE1, corresponding to the ectodomain, was expressed in recombinant, baculovirus-infected insect cells
- Purified BACE1 exists as the proBACE1 form having an apparent molecular weight of 55 kDa and an N-terminal sequence of TQHGIRLPLR.
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Content And Storage: Components of the BACE1 FRET Assay Kit are listed in Table 1. Sufficient reagent is supplied for 400 high-throughput kinetic or endpoint assays. The BACE1 Enzyme is also available separately. Store BACE1 Enzyme at -80°C. Store BACE1 Substrate and BACE1 Product Standard at -20°C. Avoid subjecting these components to repeated freeze-thaw cycles. The BACE1 Assay Buffer and BACE1 Stop Solution can be thawed upon receipt and stored at room temperature.
Product Type: FRET Assay Kit
Substrate Type: Beta-Secretase Substrate
Quantity: 400 x 40 μL Assays
Target: Beta Secretase (BACE1)
Assay: β-Secretase Assay
Substrate Properties: Peptide-Based Substrate
For Use With (Application): FRET
Shipping Condition: Dry Ice
Content And Storage:
Components of the BACE1 FRET Assay Kit are listed in Table 1. Sufficient reagent is supplied for 400 high-throughput kinetic or endpoint assays. The BACE1 Enzyme is also available separately. Store BACE1 Enzyme at -80°C. Store BACE1 Substrate and BACE1 Product Standard at -20°C. Avoid subjecting these components to repeated freeze-thaw cycles. The BACE1 Assay Buffer and BACE1 Stop Solution can be thawed upon receipt and stored at room temperature.
Product Type:
FRET Assay Kit
Substrate Type:
Beta-Secretase Substrate
Quantity:
400 x 40 μL Assays
Target:
Beta Secretase (BACE1)
Assay:
β-Secretase Assay
Substrate Properties:
Peptide-Based Substrate
For Use With (Application):
FRET
Shipping Condition:
Dry Ice
Content And Storage: Vivid™ CYP450 Screening kits include:• Vivid™ CYP450 Fluorogenic substrates (also available separately)• BACULOSOMES™ CYP450 reagents• Fluorescent standards• NADPH-regeneration systemStore reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)
Product Type: __
Substrate Type: Cytochrome P450 Substrate
Quantity: 0.1 mg
Target: __
Assay: __
Substrate Properties: Chemical Substrate
For Use With (Application): __
Shipping Condition: Approved for shipment on Wet or Dry Ice
Content And Storage:
Vivid™ CYP450 Screening kits include:• Vivid™ CYP450 Fluorogenic substrates (also available separately)• BACULOSOMES™ CYP450 reagents• Fluorescent standards• NADPH-regeneration systemStore reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)
Product Type:
__
Substrate Type:
Cytochrome P450 Substrate
Quantity:
0.1 mg
Target:
__
Assay:
__
Substrate Properties:
Chemical Substrate
For Use With (Application):
__
Shipping Condition:
Approved for shipment on Wet or Dry Ice
Content And Storage: Nuclear Receptor Buffer F may be shipped frozen or at room temperature. It should be stored at room temperature (20-30°C).
Product Type: Nuclear Receptor Buffer
Substrate Type: __
Quantity: 25 mL
Target: __
Assay: __
Substrate Properties: __
For Use With (Application): __
Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
Content And Storage:
Nuclear Receptor Buffer F may be shipped frozen or at room temperature. It should be stored at room temperature (20-30°C).
Product Type:
Nuclear Receptor Buffer
Substrate Type:
__
Quantity:
25 mL
Target:
__
Assay:
__
Substrate Properties:
__
For Use With (Application):
__
Shipping Condition:
Approved for shipment at Room Temperature or on Wet or Dry Ice
Content And Storage: The Eu-Streptavidin Kit contains 10 μg Eu-streptavidin, 400 μl AlexaFluor™ 647-biotin control peptide and 100 mL of TR-FRET Dilution Buffer. Store reagents as indicated on the certificate of analysis.
Product Type: Streptavidin Conjugate (Fluorescent)
Substrate Type: __
Quantity: 1 kit
Target: __
Assay: __
Substrate Properties: __
For Use With (Application): __
Shipping Condition: Dry Ice
Content And Storage:
The Eu-Streptavidin Kit contains 10 μg Eu-streptavidin, 400 μl AlexaFluor™ 647-biotin control peptide and 100 mL of TR-FRET Dilution Buffer. Store reagents as indicated on the certificate of analysis.
Product Type:
Streptavidin Conjugate (Fluorescent)
Substrate Type:
__
Quantity:
1 kit
Target:
__
Assay:
__
Substrate Properties:
__
For Use With (Application):
__
Shipping Condition:
Dry Ice