Thermo Scientific™ Detoxi-Gel™ Endotoxin Removing Gel

Description

• Thermo Scientific™ Detoxi-Gel Endotoxin Removing Gel and Columns provide a quick method to remove endotoxins (lipopolysaccharides or LPS) from samples
• Detoxi-Gel Resin is a beaded agarose support onto which polymixin B has been immobilized
• This ligand binds to the lipid A portion of lipopolysaccharides (LPS), which are the dominant form of endotoxins that affect biological samples
• Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be added to cells for various treatment experiments
• Detoxi-Gel Resin provides a quick method for removal of endotoxins from samples
• It is available in convenient pre-packed columns and two sizes of slurry for use in small-scale or large-scale processes
• Highlights: Affinity-based – uses immobilized polymixin B to remove endotoxins by binding to their lipid A domains High capacity – 1mL of Detoxi-Gel Resin removes at least 9995 endotoxin units from a 5mL sample containing 10,000 EU of lipopolysaccharide (LPS) Reusable – no loss in binding capacity even after 10 regenerations; requires activation and regeneration with 1% sodium deoxycholate (Part No
• 89904) Testing required – protein recovery is dependent upon protein type and concentration; empirical testing is required to validate the method for particular protein samples Versatile – remove endotoxin from protein solutions, cell culture media and pharmacological components Requires: Sodium deoxycholate (Part No
• 89904) for resin activation and regeneration.