Thermo Scientific™ Micrococcal Nuclease Solution (≥100 U/μL)

Description

• Thermo Scientific™ Micrococcal Nuclease (MNase) is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments
• This micrococcal nuclease is a stable liquid form of the enzyme derived from Staphylococcus aureus
• Micrococcal nuclease (MNase) exhibits exo- and endo-5'-phosphodiesterase activities against DNA and RNA
• This enzyme digests double-stranded, single-stranded, circular and linear nucleic acids
• The highest activity is toward single-stranded nucleic acid substrates with preference for AT- or AU-rich regions
• Enzymatic activity occurs at pH 7 to 10 and is strictly dependent on calcium for the digestion of RNA and DNA substrates
• Micrococcal nuclease is suitable for removing nucleic acids from cell lysates, releasing chromatin-bound proteins and shearing chromatin for use in chromatin immunoprecipitation (ChIP) experiments
• Highlights: Digests nucleic acids (DNA and RNA) Effective for double-stranded, single-stranded, circular and linear nucleic acids Active in neutral to alkaline buffer conditions containing divalent calcium ions Supplied at ≥100 units/μL in 10mM Tris-HCl, pH 7.5, 50mM NaCl, 1mM EDTA, 50% glycerol Compatible with: Thermo Scientific Subcellular Protein Fractionation Kit (Part No
• 78840); Thermo Scientific Pierce ChIP Kit (Part No
• 26156) Recommended for: Enzymatic DNA shearing for chromatin immunoprecipitation assays (ChIP); Removal of unwanted genomic or organellar nucleic acids from cell lysates; Degradation of nucleic acids in subcellular fractionation procedures