Thermo Scientific™ Mouse Embryonic Fibroblast Isolation Enzyme 1 (with papain) for Pierce™ Primary Cell Isolation Kits

Description

• Ensure optimal yield and viability of fibroblasts from mouse embryos with the isolation and culturing reagents in the Thermo Scientific™ Pierce™ Mouse Embryonic Fibroblast Isolation Kit
• The Thermo Scientific Pierce Mouse Embryonic Fibroblast Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of fibroblasts from mouse embryos
• The Mouse Embryonic Fibroblast Isolation Kit has been designed to obtain high viability and purity of embryonic fibroblasts using validated reagents and an optimized procedure
• The kit contains a tissue-specific dissociation enzyme and media formulated for primary cell culture that promotes the isolation growth of functional fibroblasts
• In addition, MEF isolated and cultured with Pierce MEF Isolation Kit can be cryopreserved for future use with very minimal loss of viability
• Highlights: Optimized – procedure and reagents optimized for viability, yield, purity, and ease-of-use Yield – provides a 2-fold increase in yield compared to do-it-yourself methods and other commercial kits Functional – cultured fibroblasts express the appropriate biochemical markers Includes: Complete kit includes Fibroblast Isolation Enzyme, Hanks' Balanced Salt Solution (HBSS), and DMEM for Primary Cell Isolation Applications: Generation of immortalized cell lines from transgenic mice Feeder layer for stem cells IHC and IF studies Product Details: Because it uses an optimized tissue-specific dissociation enzyme, this kit isolates fibroblasts with greater yield and cell viability than cells isolated by existing methods
• Isolated MEF express fibroblast-specific protein markers, confirming their viability and unperturbed metabolic function
• After isolation, the mouse fibroblasts may be immortalized by continuous passage for the establishment of unique cell lines
• They may also be used as a cell culture model for a diverse range of studies such as gene regulation and stem cell research [1,2,3,4].