Thermo Scientific™ Lysozyme

Description

• Lysozyme is an enzyme used to break down bacterial cell walls to improve protein or nucleic acid extraction efficiency
• Lysozymes (muramidases) are a family of enzymes with antimicrobial activity characterized by the ability to damage the cell wall of bacteria
• The enzyme acts by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycans and between the N-acetyl-D-glucosamine residues in chitodextrins
• Although hen egg white lysozyme is most effective for the lysis of gram-positive bacteria, it also facilitates the lysis of gram-negative bacteria such as Salmonella and Shigella
• The lysis of E
• coli is especially improved by the addition of both lysozyme and a nucleases such as DNase I
• Highlights: Bacterial cell wall lytic enzyme that improves inclusion body protein purification Compatible with Thermo Scientific Pierce Cell Lysis Reagents Available in two convenient formats: lyophilized powder for large volume applications (Part No
• 89833) and convenient glycerol freezer stock (Part No
• 90082) Properties of lysozyme: Enzymatic function: Catalyzes the hydrolysis of 1,4-β-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycans and between N-acetyl-D-glucosamine residues in chitodextrins Molecular weight: 14.388 kDa Extinction coefficient: ∽26.4 at 280nm Isoelectric point: pH 11.0 Unit definition: 1 unit is that amount of enzyme needed to catalyze a decrease in absorbance at 450nm of 0.001/min at 25 degrees at pH 6.24 in a 1cm cuvette due to lysis using a ∼0.25mg/mL suspension of Micrococcus luteus .