Thermo Scientific™ Pierce™ Primary Cardiomyocyte Isolation Kit

Description

• The Thermo Scientific Pierce Primary Cardiomyocyte Isolation Kit provides isolation and culturing reagents for the optimal yield and viability of primary cardiomyocytes from neonatal heart from mouse and rat
• The Primary Cardiomyocyte Isolation Kit has been designed to obtain high viability and purity of primary cardiomyocytes using validated reagents and an optimized procedure for enzymatic digestion and culturing
• The kit contains two tissue-specific dissociation enzymes, media formulated for primary cell culture, and a cardiomyocyte cell culture growth supplement that promotes the isolation, enrichment and growth of functional cardiomyocytes
• The validated protocol ensures optimal isolation and significantly reduces the processing time of isolation when compared to other methods
• Addition of the cardiomyocyte growth supplement to culture cells further enriches for cardiomycytes, and cultures have been maintained for up to 7 days using the kit components
• Highlights: Optimized - procedure and reagents optimized for viability, yield, purity, and ease-of-use Time-saving - isolation protocol requires less than 2 hours compared to up to 21 hours by other methods Yield - provides a 1.5- to 4-fold increase in yield compared to do-it-yourself methods and other commercial kits Viability - provides higher viability than do-it-yourself methods and other commercial kits Functional - cultured cardiomyocytes express the appropriate biochemical markers with validated beating activity Includes: Complete kit includes Cardiomyocyte Isolation Enzyme 1, Cardiomyocyte Isolation Enzyme 2, Hanks' Balanced Salt Solution (HBSS), DMEM for Primary Cell Isolation, and Cardiomyocyte Growth Supplement Applications: Cardiomyocyte cell differentiation Immunohistochemistry (IHC) Functional and biochemical assays Biologically relevant system for preclinical drug discovery and predictive disease modeling Product Details: Current methods for dissociation of cardiac cells from heart tissue require repeated (5 to 8 incubations) or lengthy (>16 hours) enzyme digestion, resulting in reduced yield and viability of isolated cells
• The Pierce Primary Cardiomyocyte Isolation Kit provides fully optimized reagents and a protocol that prevents overdigestion for the isolation and culture of cardiomyocytes
• The complete process from handling primary tissues to seeding cells in culture vessels can be completed within 2 hours
• Because it uses optimized tissue-specific dissociation enzymes, this kit isolates cardiomyocytes with greater yield and cell viability than cells isolated by existing methods
• The isolated primary cardiomyocytes express cardiomyocyte protein markers and exhibit contractile function, thereby providing a model system for studies of contraction, ischaemia, hypoxia and the toxicity of various compounds
• References: 1
• Louch, W.E., (2011) Methods in cardiomyocyte isolation, culture, and Gene Transfer
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• 51(3):288-98
• 2
• Chlopcikova, S., et al
• (2001) Neonatal rat cardiomyocytes – a model for the study of morphological, biochemical and electrophysiological characteristics of the heart
• Biomed
• Papers 145(2):49-55
• 3
• Gorelik, J., et al
• (2004) Comparison of arrhytmogenic effects of tauro- and glycoconjugates of cholic acid in an in vitro study of rat cardiomyocytes
• Int
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• Gynaecol., 111:867-70.