PI88890
Thermo Scientific™ 1-Step Human High-Yield IVT Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI88890-Each-of-1 | In Stock | ₹ 83,506.56 |
PI88890 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 83,506.56
GST (18%): ₹ 15,031.181
Total Price: ₹ 98,537.741
Type
1-step Human High-yield Mini IVT Kit
Quantity
2 x 100 μL
Expression System
Cell-Free
Sufficient For
2 Reactions of 100 μL Each
Content And Storage
Sufficient For: 2 reactions of 100 μL each• HeLa Lysate, 110 μL• Accessory Proteins, 25 μL• Reaction Mix, 40 μL• 5X Dialysis Buffer, 600 μL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Microdialysis Device, 2 each• Nuclease-free Water, 5 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type
Insoluble Protein
Related Products
Description
- The Thermo Scientific™ 1-Step Human High-Yield In Vitro Translation (IVT) Kits with continuous-feed device enables the expression of functional proteins with 10 to 100 times greater yield per mL than other eukaryotic IVT translation systems
- The 1-Step High-Yield IVT System uses modified HeLa cell extracts to take advantage of the robust human transcription and translation machinery and generate functional full-length proteins
- In this system, protein expression is performed in a proprietary mini dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis
- This continuous-exchange cell-free (CECF) system enables protein expression for up to 24 hours and produces protein yields ranging from 250 to 750μg/mL
- The complete kits come with all components required for proper transcription and translation of recombinant protein, including an optimized expression vector
- Highlights: High expression – up to 750μg/mL of expressed protein Reproducibility – low variability between experiments Fast – express high levels of protein with an overnight incubation Functional – obtain more active protein than with bacterial protein expression systems 1-Step Protein Expression: Streamlined reaction – requires only five components Easy-to-use – transcription and translation is performed in one reaction step Adapt for labeling – amenable to incorporation of heavy or unnatural amino acids Includes: Complete kits contain cell lysate, reaction mix, accessory proteins, 5X dialysis buffer, control DNA, cloning vector, mini dialysis devices and centrifuge tubes Requires: 30°C incubator or water bath Recommended for: Production of recombinant proteins for structural analysis; Generate active proteins for protein interaction studies; Perform rapid expression and characterization of mutant proteins; Small-scale production of virual particles; Incorporation of unnatural amino acids or toxic protein production
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Type: 1-step Human High-yield Mini IVT Kit
Quantity: 2 x 100 μL
Expression System: Cell-Free
Sufficient For: 2 Reactions of 100 μL Each
Content And Storage: Sufficient For: 2 reactions of 100 μL each• HeLa Lysate, 110 μL• Accessory Proteins, 25 μL• Reaction Mix, 40 μL• 5X Dialysis Buffer, 600 μL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Microdialysis Device, 2 each• Nuclease-free Water, 5 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type: Insoluble Protein
Type:
1-step Human High-yield Mini IVT Kit
Quantity:
2 x 100 μL
Expression System:
Cell-Free
Sufficient For:
2 Reactions of 100 μL Each
Content And Storage:
Sufficient For: 2 reactions of 100 μL each• HeLa Lysate, 110 μL• Accessory Proteins, 25 μL• Reaction Mix, 40 μL• 5X Dialysis Buffer, 600 μL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Microdialysis Device, 2 each• Nuclease-free Water, 5 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type:
Insoluble Protein
Type: 1-step Human High-yield Mini IVT Kit
Quantity: 10 x 100 μL
Expression System: Cell-Free
Sufficient For: 10 Reactions of 100 μL Each
Content And Storage: Sufficient For: 10 reactions of 100 μL each• HeLa Lysate, 500 μL• Accessory Proteins, 100 μL• Reaction Mix, 200 μL• 5X Dialysis Buffer, 3 mL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Microdialysis Device, 10 each• Nuclease-free Water, 3 x 5 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type: Insoluble Protein
Type:
1-step Human High-yield Mini IVT Kit
Quantity:
10 x 100 μL
Expression System:
Cell-Free
Sufficient For:
10 Reactions of 100 μL Each
Content And Storage:
Sufficient For: 10 reactions of 100 μL each• HeLa Lysate, 500 μL• Accessory Proteins, 100 μL• Reaction Mix, 200 μL• 5X Dialysis Buffer, 3 mL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Microdialysis Device, 10 each• Nuclease-free Water, 3 x 5 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type:
Insoluble Protein
Type: 1-step Human High-yield Maxi IVT Kit
Quantity: 2 x 2 mL
Expression System: Cell-Free
Sufficient For: 2 Reactions of 2 μL Each
Content And Storage: Sufficient For: 2 reactions of 2 mL each• HeLa Lysate, 4 x 500 μL• Accessory Proteins, 4 x 100 μL• Reaction Mix, 4 x 200 μL• 5X Dialysis Buffer, 23 mL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Maxi dialysis Device, 2 each• Nuclease-free Water, 3 x 50 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type: Insoluble Protein
Type:
1-step Human High-yield Maxi IVT Kit
Quantity:
2 x 2 mL
Expression System:
Cell-Free
Sufficient For:
2 Reactions of 2 μL Each
Content And Storage:
Sufficient For: 2 reactions of 2 mL each• HeLa Lysate, 4 x 500 μL• Accessory Proteins, 4 x 100 μL• Reaction Mix, 4 x 200 μL• 5X Dialysis Buffer, 23 mL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Maxi dialysis Device, 2 each• Nuclease-free Water, 3 x 50 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type:
Insoluble Protein
Type: __
Quantity: 10 mg
Expression System: __
Sufficient For: __
Content And Storage: Upon receipt store at -20°C protected from light and moisture.
Expressed Protein Type: __
Type:
__
Quantity:
10 mg
Expression System:
__
Sufficient For:
__
Content And Storage:
Upon receipt store at -20°C protected from light and moisture.
Expressed Protein Type:
__