PI88882
Thermo Scientific™ 1-Step Human Coupled IVT Kit - DNA
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI88882-Each-of-1 | In Stock | ₹ 1,08,746.76 |
PI88882 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,08,746.76
GST (18%): ₹ 19,574.417
Total Price: ₹ 1,28,321.177
Description
Pierce 1-Step Human Coupled IVT Kit - DNA
Sufficient For
40 Reactions of 25 μL Each
Content And Storage
Sufficient For: 40 reactions of 25 μL each• HeLa Lysate, 500 μL• Accessory Proteins, 100 μL• Reaction Mix, 200 μL• Positive Control DNA: pCFE-GFP, 10 μg• pT7CFE1-CHis, 10 μg• Nuclease-Free Water, 1.5 mLUpon receipt store kit at -80°C. Product is shipped on dry ice
Expressed Protein Type
Membrane Protein
Type
1-step Human Coupled IVT Kit
Quantity
40 Reaction Kit
Expression System
Cell-Free
Related Products
Description
- The Thermo Scientific™ 1-Step Human In Vitro Protein Expression Kits enable the translation and post-transcriptional modification of full-length proteins from mRNA or plasmid templates with yields of up to 100μg/mL per reaction
- The Human IVT Kits are unique HeLa cell lysate-based protein expression systems for in vitro translation or coupled transcription/translation reactions
- Protein expression is performed in a single 90-minute reaction that can be extended for up to 6 hours with continued protein production up to 100μg/mL when combined with the optimized pT7CFE1 Expression Vector
- The Human IVT Kits can express functional enzymes, phosphoproteins, glycoproteins and membrane proteins for immediate use in studying protein interactions, performing rapid mutational analysis and measuring activity
- Highlights: Functional – uses the human translational machinery to express active proteins Convenient – perform transcription and translation in a single step High performance – greater yields compared to rabbit reticulocyte in vitro translation Reliable – express proteins that fail in rabbit reticulocyte systems Better Than Traditional Methods: HeLa cell-free extracts are capable of expressing proteins with post-translational modifications Accurate translation delivers full-length protein compatible with downstream applications Protein translation is optimized with EMCV IRES element and other mRNA stabilizing elements Includes: Complete kits contain HeLa cell lysate, accessory proteins, reaction mix, nuclease-free water, expression vector and a GFP-positive control vector Requires: 30°C incubator or water bath Recommended for: Express proteins to measure enzyme activity; Perform mutational analysis; Express protein for use in gel mobility shift assays; Perform co-immunoprecipitation; Express cytotoxic proteins; Perform unnatural amino acid labeling
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Description: Pierce 1-Step Human Coupled IVT Kit - DNA
Sufficient For: 40 Reactions of 25 μL Each
Content And Storage: Sufficient For: 40 reactions of 25 μL each• HeLa Lysate, 500 μL• Accessory Proteins, 100 μL• Reaction Mix, 200 μL• Positive Control DNA: pCFE-GFP, 10 μg• pT7CFE1-CHis, 10 μg• Nuclease-Free Water, 1.5 mLUpon receipt store kit at -80°C. Product is shipped on dry ice
Expressed Protein Type: Membrane Protein
Type: 1-step Human Coupled IVT Kit
Quantity: 40 Reaction Kit
Expression System: Cell-Free
Description:
Pierce 1-Step Human Coupled IVT Kit - DNA
Sufficient For:
40 Reactions of 25 μL Each
Content And Storage:
Sufficient For: 40 reactions of 25 μL each• HeLa Lysate, 500 μL• Accessory Proteins, 100 μL• Reaction Mix, 200 μL• Positive Control DNA: pCFE-GFP, 10 μg• pT7CFE1-CHis, 10 μg• Nuclease-Free Water, 1.5 mLUpon receipt store kit at -80°C. Product is shipped on dry ice
Expressed Protein Type:
Membrane Protein
Type:
1-step Human Coupled IVT Kit
Quantity:
40 Reaction Kit
Expression System:
Cell-Free
Description: __
Sufficient For: 2 Reactions of 100 μL Each
Content And Storage: Sufficient For: 2 reactions of 100 μL each• HeLa Lysate, 110 μL• Accessory Proteins, 25 μL• Reaction Mix, 40 μL• 5X Dialysis Buffer, 600 μL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Microdialysis Device, 2 each• Nuclease-free Water, 5 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type: Insoluble Protein
Type: 1-step Human High-yield Mini IVT Kit
Quantity: 2 x 100 μL
Expression System: Cell-Free
Description:
__
Sufficient For:
2 Reactions of 100 μL Each
Content And Storage:
Sufficient For: 2 reactions of 100 μL each• HeLa Lysate, 110 μL• Accessory Proteins, 25 μL• Reaction Mix, 40 μL• 5X Dialysis Buffer, 600 μL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Microdialysis Device, 2 each• Nuclease-free Water, 5 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type:
Insoluble Protein
Type:
1-step Human High-yield Mini IVT Kit
Quantity:
2 x 100 μL
Expression System:
Cell-Free
Description: __
Sufficient For: 10 Reactions of 100 μL Each
Content And Storage: Sufficient For: 10 reactions of 100 μL each• HeLa Lysate, 500 μL• Accessory Proteins, 100 μL• Reaction Mix, 200 μL• 5X Dialysis Buffer, 3 mL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Microdialysis Device, 10 each• Nuclease-free Water, 3 x 5 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type: Insoluble Protein
Type: 1-step Human High-yield Mini IVT Kit
Quantity: 10 x 100 μL
Expression System: Cell-Free
Description:
__
Sufficient For:
10 Reactions of 100 μL Each
Content And Storage:
Sufficient For: 10 reactions of 100 μL each• HeLa Lysate, 500 μL• Accessory Proteins, 100 μL• Reaction Mix, 200 μL• 5X Dialysis Buffer, 3 mL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Microdialysis Device, 10 each• Nuclease-free Water, 3 x 5 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type:
Insoluble Protein
Type:
1-step Human High-yield Mini IVT Kit
Quantity:
10 x 100 μL
Expression System:
Cell-Free
Description: __
Sufficient For: 2 Reactions of 2 μL Each
Content And Storage: Sufficient For: 2 reactions of 2 mL each• HeLa Lysate, 4 x 500 μL• Accessory Proteins, 4 x 100 μL• Reaction Mix, 4 x 200 μL• 5X Dialysis Buffer, 23 mL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Maxi dialysis Device, 2 each• Nuclease-free Water, 3 x 50 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type: Insoluble Protein
Type: 1-step Human High-yield Maxi IVT Kit
Quantity: 2 x 2 mL
Expression System: Cell-Free
Description:
__
Sufficient For:
2 Reactions of 2 μL Each
Content And Storage:
Sufficient For: 2 reactions of 2 mL each• HeLa Lysate, 4 x 500 μL• Accessory Proteins, 4 x 100 μL• Reaction Mix, 4 x 200 μL• 5X Dialysis Buffer, 23 mL• Positive Control DNA (pCFE-GFP), 10 μg• pT7CFE1-NHis-GST-CHA, 10 μg• Maxi dialysis Device, 2 each• Nuclease-free Water, 3 x 50 mLStore micro-dialysis devices and nuclease-free water at room temperature. Store all other components at -80°C.
Expressed Protein Type:
Insoluble Protein
Type:
1-step Human High-yield Maxi IVT Kit
Quantity:
2 x 2 mL
Expression System:
Cell-Free