Thermo Scientific™ Pierce™ Biotinylated Protein Interaction Pull-Down Kit

Description

• Thermo Scientific™ Pierce Biotinylated Protein Interaction Pull-Down Kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand
• The kit makes it possible to perform an efficient pull-down assay with high protein recovery and reproducibility
• This protocol uses immobilized streptavidin to capture a biotinylated protein and a spin column format that enables complete gel retention during each capture, wash and elution step
• When the biotinylated bait protein is immobilized, it can withstand stringent washes and remains bound during prey protein elution, which results in an isolated prey protein without bait protein contamination
• This technique is ideal when using native or recombinant proteins that are not expressed with a fusion tag
• Additionally, prey protein is isolated from the immobilized bait protein using native binding conditions and nondenaturing elution conditions
• The Biotinylated Protein Interaction Pull-Down Kit is designed to teach the method to first-time users and to increase ease-of-use and convenience for experienced researchers
• Highlights: Biotin pull-down – purifies protein interactors of any biotinylated protein or biotin-tagged ligand Complete kit – provides all components and detailed protocol for purifying protein:protein interactions No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge) Spin column efficiency – microcentrifuge spin columns facilitate microcentrifuge spin columns facilitate simple and efficient manipulation of streptavidin agarose beads, including simple processing of multiple samples Includes: Streptavidin agarose resin, TBS pack, biotin blocking buffer, wash buffer, elution buffer, spin columns and collection tubes Recommended for: Discover a new protein interaction complex using a biotinylated protein as the bait to capture a putative binding partner from a cell lysate or other sample; Confirm expression of a known protein interaction target in a cell lysate; Determine the ability of two purified proteins to interact (bind) in various buffer conditions; Extract protein:protein interaction information from in vitro transcription/ translation lysates