PI26149
Thermo Scientific™ Pierce™ Co-Immunoprecipitation Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI26149-Each-of-1 | In Stock | ₹ 56,127.36 |
PI26149 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 56,127.36
GST (18%): ₹ 10,102.925
Total Price: ₹ 66,230.285
Content And Storage
Sufficient For: Preparing and performing 50 co-IP reactions, each with 25 μL of resin• AminoLink Plus Coupling Resin, 2 mL• Coupling Buffer (20X), 25 mL• Sodium Cyanoborohydride Solution (5M), 0.5 mL• Quenching Buffer, 50 mL• Wash Solution, 60 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Modified Dulbecco's PBS (20X), 25 mL• Conditioning Buffer (100X), 5 mL• Elution Buffer, 50 mL• Lane Marker Sample Buffer (5X), 5 mL• Pierce Spin Columns, Screw Cap, 50 columns• Microcentrifuge Collection Tubes, 2 mL, 100 tubes• Microcentrifuge Collection Tubes, 1.5 mL, 50 tubes• Pierce Control Agarose Resin, 2 mLStore at 4°C.
For Use With (Equipment)
Microcentrifuge
Quantity
1 Kit
Target
Not Target-Specific
Sufficient For
50 Reactions
Assay
Affinity Purification
Product Line
Pierce™
For Use With (Application)
Immunoprecipitation (IP)
Type
Immunoprecipitation/Co-Immunoprecipitation Kit
Format
Kit
Related Products
Description
- Thermo Scientific™ Pierce Co-Immunoprecipitation Kit provides all the components necessary to perform a properly controlled co-IP experiment and obtain purified products without antibody contamination
- The Co-Immunoprecipitation (Co-IP) Kit enables isolation of native protein complexes from a lysate or other complex mixture by directly immobilizing purified antibodies onto an agarose support
- Co-IP is a common approach to study protein:protein interactions that uses an antibody to immunoprecipitate the antigen (bait protein) and co-immunoprecipitate any interacting proteins (prey proteins)
- Traditional co-IP methods that use Protein A or G result in co-elution of the antibody heavy and light chains that may co-migrate with relevant bands, masking important results
- The Pierce Co-IP Kit resolves this issue by covalently coupling antibodies onto an amine-reactive resin
- The kit includes optimized buffers for protein binding and recovery, reagents to perform control experiments and efficient spin columns and collection tubes, which shorten the protocol and minimize handling and mixing
- Highlights: Works for any purified antibody – perform co-immunoprecipitation experiments using any purified antibody regardless of species or Ig class (chicken IgY, human IgE, mouse IgG1, IgM, etc.); no dependence on Protein A and Protein G affinity binding No antibody interference – covalent attachment method and non-reducing elution system yields pure co-IP products without contamination by the IP antibody Reusable antibody beads – covalent antibody immobilization allows prepared IP resin to be reused and provides potential savings of costly antibody Complete Kit – all reagents for antibody immobilization and many IP experiments, including binding and wash buffers, elution buffer and convenient microcentrifuge spin columns to make resin manipulations easier and more efficient Control beads included – underivatized agarose beads provided for use as negative control for nonspecific binding Versatile – co-IP method is compatible with any physiolgical (non-denaturing) protein sample buffer that is compatible with the specific antibody-antigen and protein interaction complex Includes: AminoLink Plus Coupling Resin, coupling buffer, quenching buffer, wash solution, sodium cyanoborohydride solution, IP lysis/wash buffer, conditioning buffer, Tris-buffered saline, elution buffer, lane marker sample buffer, control agarose resin, spin columns and collection tubes
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Content And Storage: Sufficient For: Preparing and performing 50 co-IP reactions, each with 25 μL of resin• AminoLink Plus Coupling Resin, 2 mL• Coupling Buffer (20X), 25 mL• Sodium Cyanoborohydride Solution (5M), 0.5 mL• Quenching Buffer, 50 mL• Wash Solution, 60 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Modified Dulbecco's PBS (20X), 25 mL• Conditioning Buffer (100X), 5 mL• Elution Buffer, 50 mL• Lane Marker Sample Buffer (5X), 5 mL• Pierce Spin Columns, Screw Cap, 50 columns• Microcentrifuge Collection Tubes, 2 mL, 100 tubes• Microcentrifuge Collection Tubes, 1.5 mL, 50 tubes• Pierce Control Agarose Resin, 2 mLStore at 4°C.
For Use With (Equipment): Microcentrifuge
Quantity: 1 Kit
Target: Not Target-Specific
Sufficient For: 50 Reactions
Assay: Affinity Purification
Product Line: Pierce™
For Use With (Application): Immunoprecipitation (IP)
Type: Immunoprecipitation/Co-Immunoprecipitation Kit
Format: Kit
Content And Storage:
Sufficient For: Preparing and performing 50 co-IP reactions, each with 25 μL of resin• AminoLink Plus Coupling Resin, 2 mL• Coupling Buffer (20X), 25 mL• Sodium Cyanoborohydride Solution (5M), 0.5 mL• Quenching Buffer, 50 mL• Wash Solution, 60 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Modified Dulbecco's PBS (20X), 25 mL• Conditioning Buffer (100X), 5 mL• Elution Buffer, 50 mL• Lane Marker Sample Buffer (5X), 5 mL• Pierce Spin Columns, Screw Cap, 50 columns• Microcentrifuge Collection Tubes, 2 mL, 100 tubes• Microcentrifuge Collection Tubes, 1.5 mL, 50 tubes• Pierce Control Agarose Resin, 2 mLStore at 4°C.
For Use With (Equipment):
Microcentrifuge
Quantity:
1 Kit
Target:
Not Target-Specific
Sufficient For:
50 Reactions
Assay:
Affinity Purification
Product Line:
Pierce™
For Use With (Application):
Immunoprecipitation (IP)
Type:
Immunoprecipitation/Co-Immunoprecipitation Kit
Format:
Kit
Content And Storage: Store at 2–8°C.
For Use With (Equipment): __
Quantity: 10 mL
Target: __
Sufficient For: __
Assay: __
Product Line: Pierce™
For Use With (Application): __
Type: Resin
Format: Bottle
Content And Storage:
Store at 2–8°C.
For Use With (Equipment):
__
Quantity:
10 mL
Target:
__
Sufficient For:
__
Assay:
__
Product Line:
Pierce™
For Use With (Application):
__
Type:
Resin
Format:
Bottle
Content And Storage: • Glycine Solution (10X), 15 mL; store at room temperature• PBS (20X), 15 mL; store at 4°C• Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 1 mL; store at 4°C• Membrane Extraction Buffer, 10 mL; store at 4°C• MNase Digestion Buffer, 10 mL; store at 4°C• DTT (7.7 mg), Lyophilized, 2 vials; store at room temperature or 4°C• MNase Stop Solution, 1 mL; store at 4°C• ChIP-Grade Protein A/G Magnetic Beads, 0.7mL; store at 4°C• IP Dilution/Wash Buffer (5X), 40 mL, store at 4°C• Sodium Chloride (5M), 6 mL; store at 4°C• IP Elution Buffer (2X), 4.5 mL; store at 4°C• Microcentrifuge Tubes, 1.5 mL, 75 tubes; store at room temperature or 4°C• DNA Clean-Up Column and Reagents, 40 purifications; store at room temperature or 4°C• DNA Clean-Up Column, 40 columns• DNA Column Binding Solution, 30 mL• DNA Column Wash Solution, 6 mL• pH Indicator, 0.8 mL• DNA Column Elution Solution, 5 mL• Micrococcal Nuclease (ChIP Grade) (10U/μL), 50 μL; store at -20°C• Anti-RNA Polymerase II Antibody, 25 μL; store at -20°C• Normal Rabbit IgG (1mg/mL), 10 μL; store at -20°C• Proteinase K (20mg/mL), 0.25 mL; store at -20°C• ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL; store at -20°C
For Use With (Equipment): __
Quantity: 30 reactions
Target: __
Sufficient For: 30 Reactions
Assay: __
Product Line: Pierce™
For Use With (Application): __
Type: ChIP Kit
Format: Kit
Content And Storage:
• Glycine Solution (10X), 15 mL; store at room temperature• PBS (20X), 15 mL; store at 4°C• Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 1 mL; store at 4°C• Membrane Extraction Buffer, 10 mL; store at 4°C• MNase Digestion Buffer, 10 mL; store at 4°C• DTT (7.7 mg), Lyophilized, 2 vials; store at room temperature or 4°C• MNase Stop Solution, 1 mL; store at 4°C• ChIP-Grade Protein A/G Magnetic Beads, 0.7mL; store at 4°C• IP Dilution/Wash Buffer (5X), 40 mL, store at 4°C• Sodium Chloride (5M), 6 mL; store at 4°C• IP Elution Buffer (2X), 4.5 mL; store at 4°C• Microcentrifuge Tubes, 1.5 mL, 75 tubes; store at room temperature or 4°C• DNA Clean-Up Column and Reagents, 40 purifications; store at room temperature or 4°C• DNA Clean-Up Column, 40 columns• DNA Column Binding Solution, 30 mL• DNA Column Wash Solution, 6 mL• pH Indicator, 0.8 mL• DNA Column Elution Solution, 5 mL• Micrococcal Nuclease (ChIP Grade) (10U/μL), 50 μL; store at -20°C• Anti-RNA Polymerase II Antibody, 25 μL; store at -20°C• Normal Rabbit IgG (1mg/mL), 10 μL; store at -20°C• Proteinase K (20mg/mL), 0.25 mL; store at -20°C• ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL; store at -20°C
For Use With (Equipment):
__
Quantity:
30 reactions
Target:
__
Sufficient For:
30 Reactions
Assay:
__
Product Line:
Pierce™
For Use With (Application):
__
Type:
ChIP Kit
Format:
Kit
Content And Storage: Upon receipt store at 4°C
For Use With (Equipment): __
Quantity: 0.65 mL
Target: IgG Antibody
Sufficient For: __
Assay: __
Product Line: Pierce™
For Use With (Application): __
Type: Agarose
Format: Bottle
Content And Storage:
Upon receipt store at 4°C
For Use With (Equipment):
__
Quantity:
0.65 mL
Target:
IgG Antibody
Sufficient For:
__
Assay:
__
Product Line:
Pierce™
For Use With (Application):
__
Type:
Agarose
Format:
Bottle