PI88828
Thermo Scientific™ Pierce™ Direct Magnetic IP/Co-IP Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI88828-Each-of-1 | In Stock | ₹ 68,405.22 |
PI88828 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 68,405.22
GST (18%): ₹ 12,312.94
Total Price: ₹ 80,718.16
Content And Storage
Sufficient For: 40 IP reactions using 25 μL of beads• Pierce NHS-Activated Magnetic Beads, 1 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Elution buffer, pH 2.0, 5 mL• Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL• Neutralization Buffer, pH 8.5, 0.5 mL• 0.67M Borate Buffer, 1 mL• BupH Borate Buffer Pack, 1 pack• Quenching Buffer, 25 mLStore at 4°C.
For Use With (Equipment)
KingFisher™ Flex, Magnetic Stand
Quantity
40 reactions
Target
Not Target-Specific
Sufficient For
40 Reactions
Assay
Affinity Purification
Product Line
Pierce™
For Use With (Application)
Pull-Down, Immunoprecipitation (IP)
Type
Immunoprecipitation/Co-Immunoprecipitation Kit
Format
Kit
Related Products
Description
- The Thermo Scientific™ Pierce Direct Magnetic IP/Co-IP Kit uses NHS-chemistry to covalently immobilize IP antibodies onto magnetic beads for effective immunoprecipitation and co-immunoprecipitation
- Magnetic IP with NHS chemistry is convenient and allows for the immobilization of IP antibody from any species and subtype without the variations in coupling efficiency seen with Protein A and Protein G
- The IP antibody is covalently attached so target proteins or co-IP protein complexes can be eluted and analyzed without antibody contamination
- The kit contains an optimized protocol and all buffers and reagents necessary for antibody coupling and high yield IP or co-IP using either manual magnetic stands or automated platforms such as the Thermo Scientific KingFisher Instruments
- Highlights: Antibody-free IP antibody is irreversibly attached to the magnetic beads, resulting in negligible co-elution of intact antibody or heavy and light chains with the purified antigen Fast complete antibody conjugation and immunoprecipitation in less than 4 hours Convenient the IP/co-IP kit contains magnetic beads and all essential buffers for optimal immunoprecipitation Antibody compatible use with any antibody species, class or isotype Low nonspecific binding the bead surface is pre-blocked and all nonreacted NHS-ester groups are fully quenched Protocol-compatible protein coupling to the beads and immunoprecipitation can be performed manually or by automation (e.g., Thermo Scientific KingFisher Instruments) Scalable use only the amount of antibody needed for a single IP experiment or prepare a larger quantity of antibody magnetic beads for multiple experiments Includes: NHS-Activated Magnetic Beads, IP lysis/wash buffer, elution buffer, neutralization buffer, quenching buffer, borate buffer and sample loading buffer
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Content And Storage: Sufficient For: 40 IP reactions using 25 μL of beads• Pierce NHS-Activated Magnetic Beads, 1 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Elution buffer, pH 2.0, 5 mL• Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL• Neutralization Buffer, pH 8.5, 0.5 mL• 0.67M Borate Buffer, 1 mL• BupH Borate Buffer Pack, 1 pack• Quenching Buffer, 25 mLStore at 4°C.
For Use With (Equipment): KingFisher™ Flex, Magnetic Stand
Quantity: 40 reactions
Target: Not Target-Specific
Sufficient For: 40 Reactions
Assay: Affinity Purification
Product Line: Pierce™
For Use With (Application): Pull-Down, Immunoprecipitation (IP)
Type: Immunoprecipitation/Co-Immunoprecipitation Kit
Format: Kit
Content And Storage:
Sufficient For: 40 IP reactions using 25 μL of beads• Pierce NHS-Activated Magnetic Beads, 1 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Elution buffer, pH 2.0, 5 mL• Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL• Neutralization Buffer, pH 8.5, 0.5 mL• 0.67M Borate Buffer, 1 mL• BupH Borate Buffer Pack, 1 pack• Quenching Buffer, 25 mLStore at 4°C.
For Use With (Equipment):
KingFisher™ Flex, Magnetic Stand
Quantity:
40 reactions
Target:
Not Target-Specific
Sufficient For:
40 Reactions
Assay:
Affinity Purification
Product Line:
Pierce™
For Use With (Application):
Pull-Down, Immunoprecipitation (IP)
Type:
Immunoprecipitation/Co-Immunoprecipitation Kit
Format:
Kit
Content And Storage: Store at 4°C
For Use With (Equipment): __
Quantity: 2 mL
Target: His
Sufficient For: __
Assay: __
Product Line: HisPur™
For Use With (Application): __
Type: Magnetic Affinity Bead
Format: __
Content And Storage:
Store at 4°C
For Use With (Equipment):
__
Quantity:
2 mL
Target:
His
Sufficient For:
__
Assay:
__
Product Line:
HisPur™
For Use With (Application):
__
Type:
Magnetic Affinity Bead
Format:
__
Content And Storage: Store at 4°C
For Use With (Equipment): __
Quantity: 10 mL
Target: His
Sufficient For: __
Assay: __
Product Line: HisPur™
For Use With (Application): __
Type: Magnetic Affinity Bead
Format: __
Content And Storage:
Store at 4°C
For Use With (Equipment):
__
Quantity:
10 mL
Target:
His
Sufficient For:
__
Assay:
__
Product Line:
HisPur™
For Use With (Application):
__
Type:
Magnetic Affinity Bead
Format:
__
Content And Storage: Store at 4°C.
For Use With (Equipment): __
Quantity: 1 mL
Target: HA
Sufficient For: __
Assay: __
Product Line: Pierce™
For Use With (Application): __
Type: Magnetic Affinity Bead
Format: __
Content And Storage:
Store at 4°C.
For Use With (Equipment):
__
Quantity:
1 mL
Target:
HA
Sufficient For:
__
Assay:
__
Product Line:
Pierce™
For Use With (Application):
__
Type:
Magnetic Affinity Bead
Format:
__