PI21277
Thermo Scientific™ Pierce™ His Protein Interaction Pull-Down Kit
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI21277-Each-of-1 | In Stock | ₹ 56,640.72 |
PI21277 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 56,640.72
GST (18%): ₹ 10,195.33
Total Price: ₹ 66,836.05
Content And Storage
Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin• Cobalt Chelate Agarose Resin, 750 μL• Pull-Down Lysis Buffer, 250 mL• Imidazole Solution (4M), 5 mL• TBS Pack (makes 500 mL), 1 pack• Pierce Spin Columns and Accessories, 27 columns• Microcentrifuge Collection Tubes, 100 tubesUpon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
For Use With (Equipment)
Microcentrifuge
Quantity
1 Kit
Target
Not Target-Specific
Sufficient For
25 Reactions
Assay
Affinity Purification
Product Line
Pierce™
For Use With (Application)
Pull-Down (Tag-Based)
Type
Pull-Down Kit
Format
Kit
Related Products
Description
- Thermo Scientific™ Pierce PolyHis Protein Interaction Pull-Down Kit purifies protein interaction complexes involving practically any user-supplied His-tagged fusion protein
- The PolyHis Protein Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with recombinant proteins containing a histidine (6xHis) fusion tag
- You provide the tagged fusion protein (bait) and cells expressing the putative protein interaction target (prey), and the pull-down kit supplies everything else: cell lysis buffer, microcentrifuge spin columns, cobalt-IMAC affinity resin (agarose beads), optimized binding and elution buffers, and a detailed protocol
- This kit is designed to teach the method to first-time users and to increase ease-of-use, convenience and reproducibility for experienced researchers
- Highlights: 6xHis pull-down – purifies protein interactors of any His-tagged fusion protein Complete kit – provides all components and detailed protocol for purifying protein:protein interactions No special equipment needed – uses common laboratory equipment and reagents (e.g., microcentrifuge) Convenient – microcentrifuge spin columns facilitate simple and efficient manipulation of cobalt IMAC agarose beads, including simple processing of multiple samples Flexible – instructions include protocols for bait and prey proteins from different sources Includes: Cobalt chelate agarose resin, lysis buffer, TBS pack, imidazole solution for elution, microcentrifuge spin columns and collection tubes Recommended for: Discover a new protein:protein interaction from a cell lysate; Confirm a putative interaction from a cell lysate or with a previously purified protein; Extract protein:protein interaction information from in vitro transcription/translation lysates
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Content And Storage: Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin• Cobalt Chelate Agarose Resin, 750 μL• Pull-Down Lysis Buffer, 250 mL• Imidazole Solution (4M), 5 mL• TBS Pack (makes 500 mL), 1 pack• Pierce Spin Columns and Accessories, 27 columns• Microcentrifuge Collection Tubes, 100 tubesUpon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
For Use With (Equipment): Microcentrifuge
Quantity: 1 Kit
Target: Not Target-Specific
Sufficient For: 25 Reactions
Assay: Affinity Purification
Product Line: Pierce™
For Use With (Application): Pull-Down (Tag-Based)
Type: Pull-Down Kit
Format: Kit
Content And Storage:
Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin• Cobalt Chelate Agarose Resin, 750 μL• Pull-Down Lysis Buffer, 250 mL• Imidazole Solution (4M), 5 mL• TBS Pack (makes 500 mL), 1 pack• Pierce Spin Columns and Accessories, 27 columns• Microcentrifuge Collection Tubes, 100 tubesUpon receipt store Pierce Lysis Buffer at room temperature. Store remaining kit components at 4°C.
For Use With (Equipment):
Microcentrifuge
Quantity:
1 Kit
Target:
Not Target-Specific
Sufficient For:
25 Reactions
Assay:
Affinity Purification
Product Line:
Pierce™
For Use With (Application):
Pull-Down (Tag-Based)
Type:
Pull-Down Kit
Format:
Kit
Content And Storage: Store desiccated at 4°C. Shipped at ambient temperature.
For Use With (Equipment): __
Quantity: 25 mg
Target: __
Sufficient For: __
Assay: __
Product Line: EZ-Link™
For Use With (Application): __
Type: __
Format: __
Content And Storage:
Store desiccated at 4°C. Shipped at ambient temperature.
For Use With (Equipment):
__
Quantity:
25 mg
Target:
__
Sufficient For:
__
Assay:
__
Product Line:
EZ-Link™
For Use With (Application):
__
Type:
__
Format:
__
Content And Storage: __
For Use With (Equipment): __
Quantity: __
Target: __
Sufficient For: __
Assay: __
Product Line: __
For Use With (Application): __
Type: __
Format: __
Content And Storage:
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For Use With (Equipment):
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Quantity:
__
Target:
__
Sufficient For:
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Assay:
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Product Line:
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For Use With (Application):
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Type:
__
Format:
__
Content And Storage: Store desiccated at -20°C. Shipped at ambient temperature.
For Use With (Equipment): __
Quantity: 25 mg
Target: __
Sufficient For: __
Assay: __
Product Line: EZ-Link™
For Use With (Application): __
Type: __
Format: __
Content And Storage:
Store desiccated at -20°C. Shipped at ambient temperature.
For Use With (Equipment):
__
Quantity:
25 mg
Target:
__
Sufficient For:
__
Assay:
__
Product Line:
EZ-Link™
For Use With (Application):
__
Type:
__
Format:
__