Thermo Scientific™ Pierce™ Glycoprotein Isolation Kit, WGA

Description

• Thermo Scientific™ Pierce Glycoprotein Isolation Kits use ConA and WGA lectin affinity resins and optimized buffers to efficiently isolate glycoproteins from serum, tissue and cultured cell lysates, thus enabling downstream analysis
• These two kits use lectins concanavilin A (ConA) and wheat germ agglutinin (WGA), respectively, as ligands to bind specific classes of glycoproteins from complex protein samples
• ConA lectin recognizes alpha-linked mannose and terminal glucose residues, while WGA lectin selectively binds to N -Acetyl glucosamine (GlcNAc) groups and to sialic acid
• The kits include ConA or WGA beaded agarose resin, optimized binding/wash and elution buffers, and easy-to-use microcentrifuge spin columns
• The kits have been used to successfully isolate glycoproteins from serum as well as HeLa and CHO cell lysates in approximately 50 minutes
• Glycosylation is a post-translational modification that plays an important role in biological functions, including immune regulation, inflammation, cell-to-cell adhesion and cell signaling
• Asparagine (N-linked) and serine/threonine residues (O-linked) are glycosylated during passage through the endoplasmic reticulum and golgi apparatus in eukaryotic and prokaryotic (i.e., Archaea and Eubacteria) systems
• Glycoconjugates are important for immune regulation, inflammation, cell-to-cell adhesion and contact inhibition, cell signaling, protection against proteolytic degradation, and other biological processes
• Highlights: High recovery – equivalent or greater glycoprotein recovery than the kits and lectin resins of other suppliers Fast – glycoprotein purification in less than one hour Versatile – isolate glycoproteins from various sample types; e.g., human serum and cell lysate Robust – lectin does not leach from resin when processing sample Convenient – complete kits include lectin resins and spin columns with all necessary reagents Compatible with Bradford-based protein assays – dialysis or protein precipitation of recovered glycoproteins is not required prior to protein assay Includes: ConA or WGA beaded agarose resin (1.1mL), binding/wash buffer, elution buffer, and microcentrifuge spin columns and accessories.