PI20475
Thermo Scientific™ AminoLink™ Plus Micro Immobilization Kit, 0.1 mL
Manufacturer: Thermo Scientific™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI20475-Each-of-1 | In Stock | ₹ 52,790.52 |
PI20475 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 52,790.52
GST (18%): ₹ 9,502.294
Total Price: ₹ 62,292.814
Capacity (Metric)
25 to 100 μg Protein Immobilization per Column
Content And Storage
Sufficient For: Immobilizing 25 to 100 μg of protein per column; Includes buffers for 20 affinity purifications• AminoLink Plus Spin Columns, 0.1 mL, 10 columns• PBS Pack (makes 500 mL), 1 pack• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Quenching Buffer, 60 mL• Wash Solution, 25 mL• Elution Buffer, 50 mL• Collection Tubes, 200 tubesStore at 4°C.
Quantity
10 Column Kit
Type
Immobilization Kit
Sufficient For
25 to 100 μg of Protein per Column
Column Type
Affinity, Agarose Resin
Formulation
Multi-component kit with 10 x 0.1mL resin columns
Product Line
AminoLink™
Target
Antibody, Proteins through Primary Amine
Stationary Phase
Aldehyde
Format
Kit, Microcentrifuge, Spin Column
Related Products
Description
- Thermo Scientific™ AminoLink Plus Coupling Resin and Kits use aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines for preparation of affinity purification columns
- The resins and kits use activated beaded agarose and a robust coupling chemistry to immobilize proteins and other ligands through primary amines (-NH 2 ) to the resin
- Once an antibody or other ligand is immobilized, the prepared affinity resin can be used for a variety of purification methods involving batch or column chromatography
- The resin and linkage are stable in binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for at least 10 rounds of affinity purification
- The AminoLink Plus Coupling Reaction involves spontaneous formation of Schiff base bonds between aldehydes (on the support) and amines (on the ligand) and their subsequent stabilization by incubation with a mild reductant (sodium cyanoborohydride)
- The entire coupling reaction, called reductive amination, occurs in 4 to 6 hours in simple non-amine buffers such as PBS
- Coupling efficiency with antibodies and typical proteins is generally greater than 85%, resulting in 1 to 20mg of immobilized protein per milliliter of agarose beads
- Highlights: AminoLink Plus Coupling Resin –aldehyde-activated crosslinked 4% beaded agarose Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH 2 ) Flexible coupling conditions – efficient ( >85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7.2) and enhanced (borate, pH 10) coupling protocols provided Stable, permanent immobilization – coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions Convenient kits and product sizes – choose 1- or 5-column kit containing complete sets of buffers, reagents and versatile spin/drip columns, or select bulk resin; bulk quantities available for manufacturing applications Includes: Kits contain AminoLink Plus Columns, neutral and enhanced coupling buffers (Part No
- 44894 only), quenching buffer, wash solution, sodium cyanoborohydride solution, and column accessories
Compare Similar Items
Show Difference
Capacity (Metric): 25 to 100 μg Protein Immobilization per Column
Content And Storage: Sufficient For: Immobilizing 25 to 100 μg of protein per column; Includes buffers for 20 affinity purifications• AminoLink Plus Spin Columns, 0.1 mL, 10 columns• PBS Pack (makes 500 mL), 1 pack• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Quenching Buffer, 60 mL• Wash Solution, 25 mL• Elution Buffer, 50 mL• Collection Tubes, 200 tubesStore at 4°C.
Quantity: 10 Column Kit
Type: Immobilization Kit
Sufficient For: 25 to 100 μg of Protein per Column
Column Type: Affinity, Agarose Resin
Formulation: Multi-component kit with 10 x 0.1mL resin columns
Product Line: AminoLink™
Target: Antibody, Proteins through Primary Amine
Stationary Phase: Aldehyde
Format: Kit, Microcentrifuge, Spin Column
Capacity (Metric):
25 to 100 μg Protein Immobilization per Column
Content And Storage:
Sufficient For: Immobilizing 25 to 100 μg of protein per column; Includes buffers for 20 affinity purifications• AminoLink Plus Spin Columns, 0.1 mL, 10 columns• PBS Pack (makes 500 mL), 1 pack• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Quenching Buffer, 60 mL• Wash Solution, 25 mL• Elution Buffer, 50 mL• Collection Tubes, 200 tubesStore at 4°C.
Quantity:
10 Column Kit
Type:
Immobilization Kit
Sufficient For:
25 to 100 μg of Protein per Column
Column Type:
Affinity, Agarose Resin
Formulation:
Multi-component kit with 10 x 0.1mL resin columns
Product Line:
AminoLink™
Target:
Antibody, Proteins through Primary Amine
Stationary Phase:
Aldehyde
Format:
Kit, Microcentrifuge, Spin Column
Capacity (Metric): 25 to 250 μg of Peptide or Protein per Reaction
Content And Storage: Sufficient For: Immobilizing 25–250 μg of peptide or protein per microcentrifuge column; Includes buffers for 20 affinity purifications• UltraLink Iodoacetyl Spin Columns, 0.1 mL, 10 columns• Coupling Buffer, 100 mL• L-Cysteine·HCl, 100 mg• Wash Solution, 25 mL• PBS Pack (makes 500 mL), 1 pack• IgG Elution Buffer, 50 mL• Collection Tubes, 200 tubesStore at 4°C protected from light.
Quantity: 1 Kit, 10 Columns
Type: Peptide Coupling Kit
Sufficient For: 10 Reactions
Column Type: Affinity, UltraLink™ Resin
Formulation: __
Product Line: UltraLink™
Target: __
Stationary Phase: Haloacetyl
Format: Kit, Microcentrifuge, Spin Column
Capacity (Metric):
25 to 250 μg of Peptide or Protein per Reaction
Content And Storage:
Sufficient For: Immobilizing 25–250 μg of peptide or protein per microcentrifuge column; Includes buffers for 20 affinity purifications• UltraLink Iodoacetyl Spin Columns, 0.1 mL, 10 columns• Coupling Buffer, 100 mL• L-Cysteine·HCl, 100 mg• Wash Solution, 25 mL• PBS Pack (makes 500 mL), 1 pack• IgG Elution Buffer, 50 mL• Collection Tubes, 200 tubesStore at 4°C protected from light.
Quantity:
1 Kit, 10 Columns
Type:
Peptide Coupling Kit
Sufficient For:
10 Reactions
Column Type:
Affinity, UltraLink™ Resin
Formulation:
__
Product Line:
UltraLink™
Target:
__
Stationary Phase:
Haloacetyl
Format:
Kit, Microcentrifuge, Spin Column
Capacity (Metric): __
Content And Storage: Upon receipt store at room temperature in sealed container to prevent oxidation. Product shipped at ambient temperature.
Quantity: 1 g
Type: __
Sufficient For: __
Column Type: __
Formulation: __
Product Line: Pierce™
Target: __
Stationary Phase: __
Format: __
Capacity (Metric):
__
Content And Storage:
Upon receipt store at room temperature in sealed container to prevent oxidation. Product shipped at ambient temperature.
Quantity:
1 g
Type:
__
Sufficient For:
__
Column Type:
__
Formulation:
__
Product Line:
Pierce™
Target:
__
Stationary Phase:
__
Format:
__
Capacity (Metric): __
Content And Storage: Upon receipt store at room temperature in sealed container to prevent oxidation. Product shipped at ambient temperature.
Quantity: 10 g
Type: __
Sufficient For: __
Column Type: __
Formulation: __
Product Line: Pierce™
Target: __
Stationary Phase: __
Format: __
Capacity (Metric):
__
Content And Storage:
Upon receipt store at room temperature in sealed container to prevent oxidation. Product shipped at ambient temperature.
Quantity:
10 g
Type:
__
Sufficient For:
__
Column Type:
__
Formulation:
__
Product Line:
Pierce™
Target:
__
Stationary Phase:
__
Format:
__