Thermo Scientific™ AminoLink™ Plus Immobilization Kit, 2 mL

Description

• Thermo Scientific™ AminoLink Plus Coupling Resin and Kits use aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines for preparation of affinity purification columns
• The resins and kits use activated beaded agarose and a robust coupling chemistry to immobilize proteins and other ligands through primary amines (-NH 2 ) to the resin
• Once an antibody or other ligand is immobilized, the prepared affinity resin can be used for a variety of purification methods involving batch or column chromatography
• The resin and linkage are stable in binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for at least 10 rounds of affinity purification
• The AminoLink Plus Coupling Reaction involves spontaneous formation of Schiff base bonds between aldehydes (on the support) and amines (on the ligand) and their subsequent stabilization by incubation with a mild reductant (sodium cyanoborohydride)
• The entire coupling reaction, called reductive amination, occurs in 4 to 6 hours in simple non-amine buffers such as PBS
• Coupling efficiency with antibodies and typical proteins is generally greater than 85%, resulting in 1 to 20mg of immobilized protein per milliliter of agarose beads
• Highlights: AminoLink Plus Coupling Resin –aldehyde-activated crosslinked 4% beaded agarose Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH 2 ) Flexible coupling conditions – efficient ( >85%) coupling over a wide range of pH (4-10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7.2) and enhanced (borate, pH 10) coupling protocols provided Stable, permanent immobilization – coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions Convenient kits and product sizes – choose 1- or 5-column kit containing complete sets of buffers, reagents and versatile spin/drip columns, or select bulk resin; bulk quantities available for manufacturing applications Includes: Kits contain AminoLink Plus Columns, neutral and enhanced coupling buffers (Part No
• 44894 only), quenching buffer, wash solution, sodium cyanoborohydride solution, and column accessories