PI44899
Thermo Scientific™ CarboxyLink™ Immobilization Kit, 2 mL
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Pack of 1 | PI44899-Pack-of-1 | In Stock | ₹ 69,389.16 |
PI44899 - Pack of 1
In Stock
Quantity
1
Base Price: ₹ 69,389.16
GST (18%): ₹ 12,490.049
Total Price: ₹ 81,879.209
Capacity (Metric)
2 mL
Content And Storage
Sufficient For: Immobilizing 1 to 2 mg of peptide per column• CarboxyLink Columns, 2 mL, 5 columns• EDC Crosslinker, 5 x 60 mg• MES Buffered Saline Pack (makes 500 mL), 1 pack• Wash Solution, 120 mL• Column AccessoriesStore EDC desiccated at -20°C. Store other components at 4°C.
Quantity
1 Kit
Type
Immobilization Kit
Sufficient For
5 Columns
Column Type
Affinity, Agarose Resin
Description
CarboxyLink Immobilization Kit, 2mL
Product Line
CarboxyLink™
Target
Antibody
Stationary Phase
Amine
Format
Spin Column
Related Products
Description
- Thermo Scientific™ CarboxyLink Coupling Resins and Kits provide for the covalent immobilization of peptides or other carboxyl-containing (-COOH) molecules to a porous, beaded resin for use in affinity purification procedures
- These activated resins enable immobilization of carboxylate ligands for use in affinity protocols
- CarboxyLink Resin is crosslinked beaded agarose (or acrylamide-based Thermo Scientific UltraLink™ Biosupport) that has been activated with diaminodipropylamine (DADPA) to contain long spacer arms, each of which have a primary amine at the end
- When incubated with the resin and the carbodiimide crosslinker EDC (included in the CarboxyLink Immobilization Kit), carboxyl-containing molecules become permanently attached to the support by stable amide bonds
- Once a molecule is coupled, the prepared affinity column can be used multiple times in typical protein affinity purification procedures
- CarboxyLink Coupling Resins also can be used to immobilize other kinds of molecules using alternative amine-reactive crosslinking chemistries
- Highlights: CarboxyLink Coupling Resins – beaded agarose or acrylamide support activated with diaminodipropylamine (DADPA) for amine-to-carboxyl conjugation using EDC crosslinker Efficient peptide immobilization – easily couple 1 to 2mg of peptide per milliliter of settled resin Stable linkage – immobilization results in covalent attachment of carboxyl groups by amide bonds, allowing for multiple rounds of affinity purification with one batch of prepared resin Flexible and gentle coupling conditions – immobilization reaction completed in simple MES or other non-amine and non-carboxyl, near-neutral buffer, with or without organic solvent Ideal for unmodified peptides – immobilizes peptides with high capacity and various orientations without steric hindrance, allowing for effective use in affinity purification of specific antibodies Convenient kits and product sizes – choose 5-column kits with complete sets of buffers, crosslinker and versatile spin/drip columns containing either type of resin (agarose or polyacrylamide) or choose stand-alone resin for other uses CarboxyLink Coupling Resin (Part No
- 20266, 44899): Ligand: diaminodipropylamine (DADPA) Support: crosslinked 4% beaded agarose Spacer arm: 9 atoms Loading: activated with at least 16μmol amine per milliliter of settled resin Format: 50% resin slurry in 0.05% sodium azide UltraLink DADPA Resin (Part No
- 53154): Ligand: diaminodipropylamine (DADPA) Support: Thermo Scientific UltraLink Biosupport Spacer arm: 14 atoms Loading: activated with at least 40μmol amine per milliliter of settled resin Format: 50% resin slurry in 0.02% sodium azide Includes: CarboxyLink Resin Columns (DADPA agarose or UltraLink Support), EDC crosslinker, coupling buffer (MES-buffered saline), wash solution, and column accessories
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Capacity (Metric): 2 mL
Content And Storage: Sufficient For: Immobilizing 1 to 2 mg of peptide per column• CarboxyLink Columns, 2 mL, 5 columns• EDC Crosslinker, 5 x 60 mg• MES Buffered Saline Pack (makes 500 mL), 1 pack• Wash Solution, 120 mL• Column AccessoriesStore EDC desiccated at -20°C. Store other components at 4°C.
Quantity: 1 Kit
Type: Immobilization Kit
Sufficient For: 5 Columns
Column Type: Affinity, Agarose Resin
Description: CarboxyLink Immobilization Kit, 2mL
Product Line: CarboxyLink™
Target: Antibody
Stationary Phase: Amine
Format: Spin Column
Capacity (Metric):
2 mL
Content And Storage:
Sufficient For: Immobilizing 1 to 2 mg of peptide per column• CarboxyLink Columns, 2 mL, 5 columns• EDC Crosslinker, 5 x 60 mg• MES Buffered Saline Pack (makes 500 mL), 1 pack• Wash Solution, 120 mL• Column AccessoriesStore EDC desiccated at -20°C. Store other components at 4°C.
Quantity:
1 Kit
Type:
Immobilization Kit
Sufficient For:
5 Columns
Column Type:
Affinity, Agarose Resin
Description:
CarboxyLink Immobilization Kit, 2mL
Product Line:
CarboxyLink™
Target:
Antibody
Stationary Phase:
Amine
Format:
Spin Column
Capacity (Metric): __
Content And Storage: Sufficient For: Purifying up to 60 mg immunoglobulin per column• Thiophilic Adsorbent Columns, 3 mL, 4 columns• Binding Buffer, 1 L• Elution Buffer, 1 L• Column Storage Buffer (2X), 100 mL• Guanidine-HCl Crystals, 230 g• Column AccessoriesStore at 4°C.
Quantity: 4 Columns
Type: __
Sufficient For: __
Column Type: Agarose Resin, Affinity
Description: Pierce Thiophilic Adsorption Kit
Product Line: Pierce™
Target: __
Stationary Phase: Sulfone Thioether
Format: Gravity Flow Column
Capacity (Metric):
__
Content And Storage:
Sufficient For: Purifying up to 60 mg immunoglobulin per column• Thiophilic Adsorbent Columns, 3 mL, 4 columns• Binding Buffer, 1 L• Elution Buffer, 1 L• Column Storage Buffer (2X), 100 mL• Guanidine-HCl Crystals, 230 g• Column AccessoriesStore at 4°C.
Quantity:
4 Columns
Type:
__
Sufficient For:
__
Column Type:
Agarose Resin, Affinity
Description:
Pierce Thiophilic Adsorption Kit
Product Line:
Pierce™
Target:
__
Stationary Phase:
Sulfone Thioether
Format:
Gravity Flow Column
Capacity (Metric): __
Content And Storage: Sufficient For: 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibodyIDA Agarose Column, 2 mL, 1 columnPolyacrylamide Desalting Column, 10 mL, 1 columnColumn Activator Buffer, 25 mLElution Buffer, 35 mLTBS Packs (each makes 500 mL), 2 packsPBS Pack (makes 500 mL), 1 packActivity Preservation Solution, 1 mLStore kit at 4°C.
Quantity: 1 kit
Type: __
Sufficient For: 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody
Column Type: __
Description: __
Product Line: Pierce™
Target: __
Stationary Phase: __
Format: __
Capacity (Metric):
__
Content And Storage:
Sufficient For: 10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibodyIDA Agarose Column, 2 mL, 1 columnPolyacrylamide Desalting Column, 10 mL, 1 columnColumn Activator Buffer, 25 mLElution Buffer, 35 mLTBS Packs (each makes 500 mL), 2 packsPBS Pack (makes 500 mL), 1 packActivity Preservation Solution, 1 mLStore kit at 4°C.
Quantity:
1 kit
Type:
__
Sufficient For:
10 purifications, each of 0.5 to 50 mg of enzyme-conjugated antibody
Column Type:
__
Description:
__
Product Line:
Pierce™
Target:
__
Stationary Phase:
__
Format:
__
Capacity (Metric): __
Content And Storage: Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Ficin Agarose, 2.5 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• Protein A Binding Buffer, 120 mL• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Quantity: 2.5 mL
Type: __
Sufficient For: __
Column Type: __
Description: __
Product Line: Pierce™
Target: __
Stationary Phase: __
Format: __
Capacity (Metric):
__
Content And Storage:
Sufficient For: 10 antibody samples, each containing 0.25 to 4 mg IgG• Pierce Ficin Agarose, 2.5 mL• Cysteine-HCl, 1 g• Mouse IgG1 Digestion Buffer, 120 mL• NAb Protein A Plus Spin Column, 1 mL, 1 column• Protein A Binding Buffer, 120 mL• IgG Elution Buffer, 120 mL• Zeba Desalt Spin Columns, 7K MWCO, 2 mL, 10 columns• Spin Columns, 0.8 mL, 10 columns• Microcentrifuge tubes, 2 mL, 30 tubesStore at 4–8°C.
Quantity:
2.5 mL
Type:
__
Sufficient For:
__
Column Type:
__
Description:
__
Product Line:
Pierce™
Target:
__
Stationary Phase:
__
Format:
__