PI20325
Thermo Scientific™ SulfoLink™ Immobilization Kit for Proteins, 2 mL
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI20325-Each-of-1 | In Stock | ₹ 29,817.66 |
PI20325 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 29,817.66
GST (18%): ₹ 5,367.179
Total Price: ₹ 35,184.839
Capacity (Metric)
2 mL
Product Line
SulfoLink™
Type
Immobilization Kit
Sufficient For
1 to 20 mg of Peptide or Protein per Column
Column Type
Affinity, Agarose Resin
Content And Storage
Sufficient For: Immobilizing 1 mg of peptide or 10 mg protein per column• SulfoLink Column, 2 mL, 1 column• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 120 mL• Wash Solution, 25 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 6 mg• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Column, 5 mL, 1 column• Column AccessoriesUpon receipt, store components at 4°C protected from light.
Quantity
1 Column Kit
Stationary Phase
Haloacetyl
Format
Spin Column
Related Products
Description
- Thermo Scientific™ SulfoLink Coupling Resin is iodoacetyl-activated for the covalent immobilization of sulfhydryl-containing peptides or proteins for affinity purification
- SulfoLink Coupling Resin is porous, crosslinked 6% beaded agarose that has been activated with iodoacetyl groups
- When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin
- The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest
- Complete SulfoLink Immobilization Kits are available for peptides and proteins
- Each kit contains all the reagents for preparing the respective peptide or protein sample and five columns containing 2mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification
- A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately
- Highlights: Specific conjugation through sulfhydryl (-SH) groups – the iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds Separate kits optimized for peptides or proteins – kits include optimized reagents for preparing peptide or protein samples for efficient immobilization Fast – spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins) Flexible coupling conditions – use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction Easy-to-follow instructions – streamlined protocols for sample preparation, immobilization and affinity purification High capacity – immobilize 1 to 2mg peptide or 2 to 20mg protein per 2mL column of SulfoLink Coupling Resin Includes: Kits contain SulfoLink Resin Columns (2mL), protein or peptide preparation reagents (e.g., reducing agent), coupling buffer, wash solution, L-cysteine solution (for quenching) and desalting columns (protein kit only) Recommended for: Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation; Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
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Capacity (Metric): 2 mL
Product Line: SulfoLink™
Type: Immobilization Kit
Sufficient For: 1 to 20 mg of Peptide or Protein per Column
Column Type: Affinity, Agarose Resin
Content And Storage: Sufficient For: Immobilizing 1 mg of peptide or 10 mg protein per column• SulfoLink Column, 2 mL, 1 column• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 120 mL• Wash Solution, 25 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 6 mg• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Column, 5 mL, 1 column• Column AccessoriesUpon receipt, store components at 4°C protected from light.
Quantity: 1 Column Kit
Stationary Phase: Haloacetyl
Format: Spin Column
Capacity (Metric):
2 mL
Product Line:
SulfoLink™
Type:
Immobilization Kit
Sufficient For:
1 to 20 mg of Peptide or Protein per Column
Column Type:
Affinity, Agarose Resin
Content And Storage:
Sufficient For: Immobilizing 1 mg of peptide or 10 mg protein per column• SulfoLink Column, 2 mL, 1 column• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 120 mL• Wash Solution, 25 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 6 mg• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Column, 5 mL, 1 column• Column AccessoriesUpon receipt, store components at 4°C protected from light.
Quantity:
1 Column Kit
Stationary Phase:
Haloacetyl
Format:
Spin Column
Capacity (Metric): __
Product Line: Pierce™
Type: __
Sufficient For: 12 to 19 mg Human IgG/mL Resin, 6 to 8mg mouse IgG/mL Resin
Column Type: Agarose Resin, Affinity
Content And Storage: Upon receipt store product at 4°C to 8°C. Product is shipped at ambient temperature.
Quantity: 5 mL
Stationary Phase: Protein A
Format: Bottle
Capacity (Metric):
__
Product Line:
Pierce™
Type:
__
Sufficient For:
12 to 19 mg Human IgG/mL Resin, 6 to 8mg mouse IgG/mL Resin
Column Type:
Agarose Resin, Affinity
Content And Storage:
Upon receipt store product at 4°C to 8°C. Product is shipped at ambient temperature.
Quantity:
5 mL
Stationary Phase:
Protein A
Format:
Bottle
Capacity (Metric): __
Product Line: Pierce™
Type: __
Sufficient For: 12 to 19 mg Human IgG/mL Resin, 6 to 8mg mouse IgG/mL Resin
Column Type: Agarose Resin, Affinity
Content And Storage: Upon receipt store product at 4°C to 8°C. Product is shipped at ambient temperature.
Quantity: 25 mL
Stationary Phase: Protein A
Format: Bottle
Capacity (Metric):
__
Product Line:
Pierce™
Type:
__
Sufficient For:
12 to 19 mg Human IgG/mL Resin, 6 to 8mg mouse IgG/mL Resin
Column Type:
Agarose Resin, Affinity
Content And Storage:
Upon receipt store product at 4°C to 8°C. Product is shipped at ambient temperature.
Quantity:
25 mL
Stationary Phase:
Protein A
Format:
Bottle
Capacity (Metric): 4000 to 6000 EU Binding per mL Gel
Product Line: Detoxi-Gel™
Type: Endotoxin Removing Gel
Sufficient For: 1 mL of gel binds 4000-6000 EU
Column Type: Affinity, Agarose Resin
Content And Storage: Store at 4°C.
Quantity: 10 mL
Stationary Phase: Polymixin B
Format: Bottle
Capacity (Metric):
4000 to 6000 EU Binding per mL Gel
Product Line:
Detoxi-Gel™
Type:
Endotoxin Removing Gel
Sufficient For:
1 mL of gel binds 4000-6000 EU
Column Type:
Affinity, Agarose Resin
Content And Storage:
Store at 4°C.
Quantity:
10 mL
Stationary Phase:
Polymixin B
Format:
Bottle