PI44893
Thermo Scientific™ Pierce™ Protein A IgG Plus Orientation Kit, 2 mL
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI44893-Each-of-1 | In Stock | ₹ 83,121.54 |
PI44893 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 83,121.54
GST (18%): ₹ 14,961.877
Total Price: ₹ 98,083.417
Capacity (Metric)
2 mL
Product Line
Pierce™
Type
IgG Plus Orientation Kit
Sufficient For
2 to 10 mg of Rabbit IgG per Column
Column Type
Affinity, Agarose Resin
Content And Storage
Sufficient For: Immobilizing 2 to 10 mg of rabbit IgG per column• Recombinant Protein A Columns, 2 mL, 2 columns• Disuccinimidyl Suberate (DSS), 2 x 13 mg• PBS Pack (makes 500 mL), 1 pack• Blocking Buffer, 6 mL• IgG Elution Buffer, 15 mL• Antibody Binding/Wash Buffer, 120 mL• Column AccessoriesStore at 4°C.
Quantity
2 columns
Stationary Phase
Protein A
Format
Spin Column
Related Products
Description
- Thermo Scientific™ Pierce IgG Plus Orientation Kits bind and crosslink antibody to Protein A or Protein G agarose resin, thereby creating a reusable affinity column for purification of antigens
- These IgG orientation kits use a crosslinker to covalently attach antibody that is bound to Protein A or Protein G agarose resin, thereby creating a reusable affinity column for purification of antigens
- The Plus Orientation Kits provide for more efficient and stable antibody immobilization than the traditional orientation method because they use the NHS-ester crosslinker DSS (disuccinimidyl suberate) rather than the imidoester crosslinker DMP (dimethyl pimelimidate)
- Several milligrams of IgG can be bound and crosslinked to each 2mL column, afterwhich the prepared affinity column can be used multiple times for antigen purification using typical binding and elution conditions
- The immobilization method is called orientation because antibodies are first captured to the affinity resin through their Fc regions, which are the target binding sites of Protein A and Protein G ligands
- Thus, the antibodies are bound with their antigen-binding sites oriented outward
- DSS crosslinker reacts with primary amines (side chain of lysine residues) of the Protein A/G and affinity-bound antibody, thereby covalently and permanently attaching the proteins together
- Highlights: Contaminant-free antigen purification – enables antigen purification without contamination (co-elution) of antibody Covalent, leak-resistant antibody immobilization – high-capacity binding of IgG to Protein A or Protein G agarose, followed by efficient crosslinking with DSS crosslinker High-quality agarose resin – crosslinked 6% beaded agarose with recombinant Protein A or Protein G Gentle binding and crosslinking conditions – coupling antibody in standard PBS Oriented immobilization – binds and crosslinks IgG with the antigen-binding sites directed outward, able to capture passing antigen in the mobile phase without steric hindrance Complete kits – include all the reagents needed to immobilize the antibody Better than DMP – less antibody leaching than traditional orientation method with dimethyl pimelimidate crosslinker Includes: Protein A or G columns, disuccinimidyl suberate (DSS), PBS pack, blocking buffer, binding/wash buffer, elution buffer, and column accessories
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Capacity (Metric): 2 mL
Product Line: Pierce™
Type: IgG Plus Orientation Kit
Sufficient For: 2 to 10 mg of Rabbit IgG per Column
Column Type: Affinity, Agarose Resin
Content And Storage: Sufficient For: Immobilizing 2 to 10 mg of rabbit IgG per column• Recombinant Protein A Columns, 2 mL, 2 columns• Disuccinimidyl Suberate (DSS), 2 x 13 mg• PBS Pack (makes 500 mL), 1 pack• Blocking Buffer, 6 mL• IgG Elution Buffer, 15 mL• Antibody Binding/Wash Buffer, 120 mL• Column AccessoriesStore at 4°C.
Quantity: 2 columns
Stationary Phase: Protein A
Format: Spin Column
Capacity (Metric):
2 mL
Product Line:
Pierce™
Type:
IgG Plus Orientation Kit
Sufficient For:
2 to 10 mg of Rabbit IgG per Column
Column Type:
Affinity, Agarose Resin
Content And Storage:
Sufficient For: Immobilizing 2 to 10 mg of rabbit IgG per column• Recombinant Protein A Columns, 2 mL, 2 columns• Disuccinimidyl Suberate (DSS), 2 x 13 mg• PBS Pack (makes 500 mL), 1 pack• Blocking Buffer, 6 mL• IgG Elution Buffer, 15 mL• Antibody Binding/Wash Buffer, 120 mL• Column AccessoriesStore at 4°C.
Quantity:
2 columns
Stationary Phase:
Protein A
Format:
Spin Column
Capacity (Metric): 2 to 40 mg of protein Immobilization per Column
Product Line: AminoLink™
Type: Immobilization Kit
Sufficient For: 2 to 40 mg of Protein per Column
Column Type: Affinity, Agarose Resin
Content And Storage: Sufficient For: Immobilizing 2 to 40 mg of protein per column• AminoLink Plus Columns, 2 mL, 5 columns• PBS Pack (makes 500mL), 1 pack• Citrate-Carbonate Buffer Pack (makes 500mL), 1 pack• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Quenching Buffer, 60 mL• Wash Solution, 240 mL• Column AccessoriesStore at 4°C
Quantity: 5 Column Kit
Stationary Phase: Aldehyde
Format: Spin Column
Capacity (Metric):
2 to 40 mg of protein Immobilization per Column
Product Line:
AminoLink™
Type:
Immobilization Kit
Sufficient For:
2 to 40 mg of Protein per Column
Column Type:
Affinity, Agarose Resin
Content And Storage:
Sufficient For: Immobilizing 2 to 40 mg of protein per column• AminoLink Plus Columns, 2 mL, 5 columns• PBS Pack (makes 500mL), 1 pack• Citrate-Carbonate Buffer Pack (makes 500mL), 1 pack• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Quenching Buffer, 60 mL• Wash Solution, 240 mL• Column AccessoriesStore at 4°C
Quantity:
5 Column Kit
Stationary Phase:
Aldehyde
Format:
Spin Column
Capacity (Metric): __
Product Line: Pierce™
Type: __
Sufficient For: __
Column Type: Agarose Resin, Affinity
Content And Storage: Sufficient For: 10 IgM purifications, each from 0.5 mL mouse ascites samples• MBP Agarose Column, 5 mL, 1 column• IgM Binding Buffer, 800 mL• IgM Elution Buffer, 500 mL• MBP Column Preparation Buffer, 50 mL• Column ExtenderStore at 4°C
Quantity: 1 Column
Stationary Phase: Lectin
Format: Kit
Capacity (Metric):
__
Product Line:
Pierce™
Type:
__
Sufficient For:
__
Column Type:
Agarose Resin, Affinity
Content And Storage:
Sufficient For: 10 IgM purifications, each from 0.5 mL mouse ascites samples• MBP Agarose Column, 5 mL, 1 column• IgM Binding Buffer, 800 mL• IgM Elution Buffer, 500 mL• MBP Column Preparation Buffer, 50 mL• Column ExtenderStore at 4°C
Quantity:
1 Column
Stationary Phase:
Lectin
Format:
Kit
Capacity (Metric): 2 mL
Product Line: CarboxyLink™
Type: Immobilization Kit
Sufficient For: 5 Columns
Column Type: Affinity, Agarose Resin
Content And Storage: Sufficient For: Immobilizing 1 to 2 mg of peptide per column• CarboxyLink Columns, 2 mL, 5 columns• EDC Crosslinker, 5 x 60 mg• MES Buffered Saline Pack (makes 500 mL), 1 pack• Wash Solution, 120 mL• Column AccessoriesStore EDC desiccated at -20°C. Store other components at 4°C.
Quantity: 1 Kit
Stationary Phase: Amine
Format: Spin Column
Capacity (Metric):
2 mL
Product Line:
CarboxyLink™
Type:
Immobilization Kit
Sufficient For:
5 Columns
Column Type:
Affinity, Agarose Resin
Content And Storage:
Sufficient For: Immobilizing 1 to 2 mg of peptide per column• CarboxyLink Columns, 2 mL, 5 columns• EDC Crosslinker, 5 x 60 mg• MES Buffered Saline Pack (makes 500 mL), 1 pack• Wash Solution, 120 mL• Column AccessoriesStore EDC desiccated at -20°C. Store other components at 4°C.
Quantity:
1 Kit
Stationary Phase:
Amine
Format:
Spin Column