PI44995
Thermo Scientific™ SulfoLink™ Immobilization Kit for Proteins, 2 mL
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI44995-Each-of-1 | In Stock | ₹ 38,416.44 |
PI44995 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,416.44
GST (18%): ₹ 6,914.959
Total Price: ₹ 45,331.399
Capacity (Metric)
2 mL
Product Line
SulfoLink™
Type
Immobilization Kit
Sufficient For
2 to 20 mg of Antibody per Column
Column Type
Affinity, Agarose Resin
Content And Storage
Sufficient For: Immobilizing 1 to 10 mg of antibody per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 500 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 5 x 6 mg• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Columns, 5 mL, 5 columns• Column AccessoriesStore at 4°C protected from light.
Quantity
5 Column Kit
Stationary Phase
Haloacetyl
Format
Spin Column
Related Products
Description
- Thermo Scientific™ SulfoLink Coupling Resin is iodoacetyl-activated for the covalent immobilization of sulfhydryl-containing peptides or proteins for affinity purification
- SulfoLink Coupling Resin is porous, crosslinked 6% beaded agarose that has been activated with iodoacetyl groups
- When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin
- The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest
- Complete SulfoLink Immobilization Kits are available for peptides and proteins
- Each kit contains all the reagents for preparing the respective peptide or protein sample and five columns containing 2mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification
- A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately
- Highlights: Specific conjugation through sulfhydryl (-SH) groups – the iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds Separate kits optimized for peptides or proteins – kits include optimized reagents for preparing peptide or protein samples for efficient immobilization Fast – spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins) Flexible coupling conditions – use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction Easy-to-follow instructions – streamlined protocols for sample preparation, immobilization and affinity purification High capacity – immobilize 1 to 2mg peptide or 2 to 20mg protein per 2mL column of SulfoLink Coupling Resin Includes: Kits contain SulfoLink Resin Columns (2mL), protein or peptide preparation reagents (e.g., reducing agent), coupling buffer, wash solution, L-cysteine solution (for quenching) and desalting columns (protein kit only) Recommended for: Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation; Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification
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Capacity (Metric): 2 mL
Product Line: SulfoLink™
Type: Immobilization Kit
Sufficient For: 2 to 20 mg of Antibody per Column
Column Type: Affinity, Agarose Resin
Content And Storage: Sufficient For: Immobilizing 1 to 10 mg of antibody per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 500 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 5 x 6 mg• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Columns, 5 mL, 5 columns• Column AccessoriesStore at 4°C protected from light.
Quantity: 5 Column Kit
Stationary Phase: Haloacetyl
Format: Spin Column
Capacity (Metric):
2 mL
Product Line:
SulfoLink™
Type:
Immobilization Kit
Sufficient For:
2 to 20 mg of Antibody per Column
Column Type:
Affinity, Agarose Resin
Content And Storage:
Sufficient For: Immobilizing 1 to 10 mg of antibody per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Preparation Buffer, 7.5 mL• SulfoLink Coupling Buffer, 500 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• 2-Mercaptoethylamine·HCl, 5 x 6 mg• L-Cysteine·HCl, 100 mg• Zeba Spin Desalting Columns, 5 mL, 5 columns• Column AccessoriesStore at 4°C protected from light.
Quantity:
5 Column Kit
Stationary Phase:
Haloacetyl
Format:
Spin Column
Capacity (Metric): 2 mL
Product Line: SulfoLink™
Type: Immobilization Kit
Sufficient For: 1 to 2 mg of Sulfhydryl-Peptide per Column
Column Type: Affinity, Agarose Resin
Content And Storage: Sufficient For: Immobilizing 0.1 to 1 mg of sulfhydryl-peptide per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Coupling Buffer, 120 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL• L-Cysteine·HCl, 100 mg• Column AccessoriesStore at 4°C protected from light.
Quantity: 5 Column Kit
Stationary Phase: Haloacetyl
Format: Spin Column
Capacity (Metric):
2 mL
Product Line:
SulfoLink™
Type:
Immobilization Kit
Sufficient For:
1 to 2 mg of Sulfhydryl-Peptide per Column
Column Type:
Affinity, Agarose Resin
Content And Storage:
Sufficient For: Immobilizing 0.1 to 1 mg of sulfhydryl-peptide per column• SulfoLink Columns, 2 mL, 5 columns• SulfoLink Coupling Buffer, 120 mL• Wash Solution, 120 mL• PBS Pack (makes 500 mL), 1 pack• Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL• L-Cysteine·HCl, 100 mg• Column AccessoriesStore at 4°C protected from light.
Quantity:
5 Column Kit
Stationary Phase:
Haloacetyl
Format:
Spin Column
Capacity (Metric): __
Product Line: Pierce™
Type: __
Sufficient For: __
Column Type: Agarose Resin, Affinity
Content And Storage: Upon receipt store at 4°C
Quantity: 1 Plate
Stationary Phase: Protein A
Format: 96-well Filter Plate
Capacity (Metric):
__
Product Line:
Pierce™
Type:
__
Sufficient For:
__
Column Type:
Agarose Resin, Affinity
Content And Storage:
Upon receipt store at 4°C
Quantity:
1 Plate
Stationary Phase:
Protein A
Format:
96-well Filter Plate
Capacity (Metric): __
Product Line: Pierce™
Type: __
Sufficient For: __
Column Type: Agarose Resin, Affinity
Content And Storage: Upon receipt store at 4°C
Quantity: 1 Plate
Stationary Phase: Protein G
Format: 96-well Filter Plate
Capacity (Metric):
__
Product Line:
Pierce™
Type:
__
Sufficient For:
__
Column Type:
Agarose Resin, Affinity
Content And Storage:
Upon receipt store at 4°C
Quantity:
1 Plate
Stationary Phase:
Protein G
Format:
96-well Filter Plate