PI44890
Thermo Scientific™ AminoLink™ Immobilization Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Pack of 1 | PI44890-Pack-of-1 | In Stock | ₹ 44,533.98 |
PI44890 - Pack of 1
In Stock
Quantity
1
Base Price: ₹ 44,533.98
GST (18%): ₹ 8,016.116
Total Price: ₹ 52,550.096
Description
AminoLink Immobilization Kit, 2mL
Product Line
AminoLink™
Target
Antibody
Stationary Phase
Aldehyde
Format
Spin Column
Content And Storage
Sufficient For: Immobilizing 2 to 20 mg of protein per column• AminoLink Columns, 2mL, 5 columns• AminoLink Coupling Buffer (pH 7.0), 250 mL• Quenching Buffer, 50 mL• Wash Buffer, 240 mL• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Column AccessoriesStore at 4°C
Quantity
5 Column Kit
Type
Immobilization Kit
Sufficient For
5 Protein Immobilizations
Column Type
Affinity, Agarose Resin
Related Products
Description
- Thermo Scientific™ AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups to enable covalent immobilization of antibodies and other proteins through primary amines
- AminoLink Coupling Resin is crosslinked 4% beaded agarose that has been activated with aldehyde groups
- Proteins and other molecules with primary amines can be covalently attached (immobilized) to the resin to make chromatography columns for use in affinity purification
- The aldehyde groups form stable secondary amine bonds with primary amines such as exist in the side chain of lysine (K) residues, which are generally abundant and readily accessible in proteins
- Once a protein is immobilized, the prepared affinity resin can be used for a variety of batch and column affinity purification methods involving binding interactions with the immobilized protein
- The resin and linkage are stable in most binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for multiple rounds of affinity purification procedures
- Highlights: AminoLink Coupling Resin – aldehyde-activated crosslinked 4% beaded agarose Ideal for antibodies and other proteins – immobilize molecules via primary amines (-NH 2 ) Flexible coupling conditions – efficient (>85%) coupling over a wide range of pH (4 to 10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent Stable, permanent immobilization – coupling reaction results in stable, leak-resistant secondary amine bond between resin and ligand Better than immobilization to CNBr-activated agarose – bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures Versatile and reusable – prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions Includes: Kit contains pre-packed AminoLink Columns, coupling buffer, quenching buffer, wash solution, sodium cyanoborohydride solution, and column accessories
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Description: AminoLink Immobilization Kit, 2mL
Product Line: AminoLink™
Target: Antibody
Stationary Phase: Aldehyde
Format: Spin Column
Content And Storage: Sufficient For: Immobilizing 2 to 20 mg of protein per column• AminoLink Columns, 2mL, 5 columns• AminoLink Coupling Buffer (pH 7.0), 250 mL• Quenching Buffer, 50 mL• Wash Buffer, 240 mL• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Column AccessoriesStore at 4°C
Quantity: 5 Column Kit
Type: Immobilization Kit
Sufficient For: 5 Protein Immobilizations
Column Type: Affinity, Agarose Resin
Description:
AminoLink Immobilization Kit, 2mL
Product Line:
AminoLink™
Target:
Antibody
Stationary Phase:
Aldehyde
Format:
Spin Column
Content And Storage:
Sufficient For: Immobilizing 2 to 20 mg of protein per column• AminoLink Columns, 2mL, 5 columns• AminoLink Coupling Buffer (pH 7.0), 250 mL• Quenching Buffer, 50 mL• Wash Buffer, 240 mL• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Column AccessoriesStore at 4°C
Quantity:
5 Column Kit
Type:
Immobilization Kit
Sufficient For:
5 Protein Immobilizations
Column Type:
Affinity, Agarose Resin
Description: __
Product Line: AminoLink™
Target: __
Stationary Phase: __
Format: __
Content And Storage: Store desiccated at room temperature. Shipped at ambient temperature
Quantity: 2 x 1 g
Type: Resin
Sufficient For: __
Column Type: __
Description:
__
Product Line:
AminoLink™
Target:
__
Stationary Phase:
__
Format:
__
Content And Storage:
Store desiccated at room temperature. Shipped at ambient temperature
Quantity:
2 x 1 g
Type:
Resin
Sufficient For:
__
Column Type:
__
Description: __
Product Line: Pierce™
Target: __
Stationary Phase: Protein A
Format: Spin Column
Content And Storage: Sufficient For: Immobilizing 2 to 10 mg of rabbit IgG per column• Recombinant Protein A Columns, 2 mL, 2 columns• Disuccinimidyl Suberate (DSS), 2 x 13 mg• PBS Pack (makes 500 mL), 1 pack• Blocking Buffer, 6 mL• IgG Elution Buffer, 15 mL• Antibody Binding/Wash Buffer, 120 mL• Column AccessoriesStore at 4°C.
Quantity: 2 columns
Type: IgG Plus Orientation Kit
Sufficient For: 2 to 10 mg of Rabbit IgG per Column
Column Type: Affinity, Agarose Resin
Description:
__
Product Line:
Pierce™
Target:
__
Stationary Phase:
Protein A
Format:
Spin Column
Content And Storage:
Sufficient For: Immobilizing 2 to 10 mg of rabbit IgG per column• Recombinant Protein A Columns, 2 mL, 2 columns• Disuccinimidyl Suberate (DSS), 2 x 13 mg• PBS Pack (makes 500 mL), 1 pack• Blocking Buffer, 6 mL• IgG Elution Buffer, 15 mL• Antibody Binding/Wash Buffer, 120 mL• Column AccessoriesStore at 4°C.
Quantity:
2 columns
Type:
IgG Plus Orientation Kit
Sufficient For:
2 to 10 mg of Rabbit IgG per Column
Column Type:
Affinity, Agarose Resin
Description: __
Product Line: AminoLink™
Target: Antibody, Proteins through Primary Amine
Stationary Phase: Aldehyde
Format: Spin Column
Content And Storage: Sufficient For: Immobilizing 2 to 40 mg of protein per column• AminoLink Plus Columns, 2 mL, 5 columns• PBS Pack (makes 500mL), 1 pack• Citrate-Carbonate Buffer Pack (makes 500mL), 1 pack• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Quenching Buffer, 60 mL• Wash Solution, 240 mL• Column AccessoriesStore at 4°C
Quantity: 5 Column Kit
Type: Immobilization Kit
Sufficient For: 2 to 40 mg of Protein per Column
Column Type: Affinity, Agarose Resin
Description:
__
Product Line:
AminoLink™
Target:
Antibody, Proteins through Primary Amine
Stationary Phase:
Aldehyde
Format:
Spin Column
Content And Storage:
Sufficient For: Immobilizing 2 to 40 mg of protein per column• AminoLink Plus Columns, 2 mL, 5 columns• PBS Pack (makes 500mL), 1 pack• Citrate-Carbonate Buffer Pack (makes 500mL), 1 pack• Sodium Cyanoborohydride Solution, 5M, 0.5 mL• Quenching Buffer, 60 mL• Wash Solution, 240 mL• Column AccessoriesStore at 4°C
Quantity:
5 Column Kit
Type:
Immobilization Kit
Sufficient For:
2 to 40 mg of Protein per Column
Column Type:
Affinity, Agarose Resin