Thermo Scientific™ GlycoLink™ Micro Immobilization Kit

Description

• Thermo Scientific™ GlycoLink Immobilization Kits provide affinity columns and reagents to immobilize glycoproteins through oxidized sugar groups for use in affinity-purification applications or studies of protein-protein interactions
• The GlycoLink Kits use hydrazide-activated resin and a special catalyst to prepare affinity columns with polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions
• Any glycosylated proteins (even monoclonal antibodies) that contain periodate-oxidizable carbohydrate groups can be immobilized with these kits to make reusable affinity columns
• Antibodies immobilized by this hydrazide immobilization chemistry have unobstructed antigen-binding sites and optimal purification capability
• Prepared columns with stable glycoproteins or antibodies can be regenerated and reused at least five times for affinity-purification without significant loss in binding capacity
• Highlights: High-capacity – immobilize 1 to 10mg of oxidized antibody or other glycoprotein per milliliter of resin Fast and efficient – immobilize in as few as 30 minutes; achieve at least 90% coupling of most glycoproteins in less than 4 hours (efficiency depends on the amount and type of glycosylation) Specific immobilization – the hydrazide-activated UltraLink Resin conjugates only to purified glycoproteins whose sugar (e.g., sialic acid) groups have been gently oxidized with periodate Stable attachment – resonance structure of the hydrazone bonds are sufficiently stable for multiple affinity purifications with one batch of prepared resin; no stabilization step is required Preserves antibody function – immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target Convenient sizes – choose from two complete 10-column kits: one with 1mL columns and one with 0.1mL columns UltraLink Hydrazide Resin: Ligand: hydrazide Support: Thermo Scientific UltraLink Biosupport Spacer arm: 22 atoms Loading: activated with at least 15μmol hydrazide per milliliter of settled resin Format: 50% resin slurry in 0.02% sodium azide Includes: Kits contain GlycoLink Columns; coupling buffer, coupling catalyst and wash buffer; oxidizing reagent; desalting columns, PBS buffer pack; and column accessories Recommended for: Purify antigens using covalently coupled antibodies; Determine binding partners for a glycoprotein of interest; Perform large-scale affinity purifications