PI88943
Thermo Scientific™ GlycoLink™ IP Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI88943-Each-of-1 | In Stock | ₹ 70,073.64 |
PI88943 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 70,073.64
GST (18%): ₹ 12,613.255
Total Price: ₹ 82,686.895
Capacity (Metric)
2 to 10 μg Oxidized Antibody or Glycoprotein Coupling to 20 μL Resin
Assay
Affinity Purification
Product Line
GlycoLink™
For Use With (Application)
Immunoprecipitation (IP)
Type
Immunoprecipitation/Co-Immunoprecipitation Kit
Format
Kit
Content And Storage
Sufficient For: 25 IP/Co-IP reactions, each using 20 μL antibody-coupled resin• UltraLink Hydrazide Resin, 0.5 mL• GlycoLink Coupling Buffer, 50 mL• Aniline, 0.2 mL• Sodium meta-periodate, 0.5 g• Wash Buffer, 60 mL• Zeba Spin Desalting Columns,0.5 mL, 25 columns• IP Lysis/Wash Buffer, 50 mL• PBS Pack (makes 500 mL), 1 pack• Elution Buffer, pH 2.8, 50 mL• Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL• Pierce Spin Columns-Screw Caps, 25 columns• Collection Tubes, 2 mL, 100 tubesStore at 4°C.
For Use With (Equipment)
Microcentrifuge
Quantity
1 Kit
Target
Not Target-Specific
Sufficient For
25 Reactions
Related Products
Description
- The Thermo Scientific GlycoLink IP Kit provides components for effective immunoprecipitation (IP and co-IP) based on small-scale, covalent, affinity-resin immobilization of antibodies and other glycoproteins via oxidized sugar groups
- The GlycoLink Kit uses hydrazide-activated UltraLink Resin to immobilize and perform 25 IP or Co-IP assays with different antibodies or glycoprotein ligands
- The method is ideal for polyclonal antibodies, which typically have abundant carbohydrates (glycosylation) on their Fc portions
- Monoclonal antibodies that contain adequate carbohydrates are also effective
- Antibodies immobilized by this hydrazide-to-carbohydrate method have unobstructed antigen-binding sites and optimal purification capability
- The prepared glycoprotein or antibody columns can be regenerated and reused at least five times for immunoprecipitation, co-immunoprecipitation or pull-down affinity-purification without significant loss in binding capacity
- Highlights: Efficient immobilization – couple 2 to 10μg of oxidized antibody or other glycoprotein to 20μL of resin in 2 hours or less Stable immobilization – resonance structure of the hydrazone bonds are sufficiently stable so the antibody remains coupled during elution Preserves binding function – immobilizes IgG via the Fc region, thereby keeping both antigen binding sites available for capturing target Versatile applications – immobilize any molecule that contains oxidizable sugars, including glycoproteins for use in purifying protein interaction binding partners Includes: Kit contains resin, buffers and reagents for immobilization; desalting columns; IP lysis/wash and elution buffers for immunoprecipitation; microcentrifuge spin columns and collection tubes; and loading buffer for SDS-PAGE Recommended for: Immunoprecipitation followed by electrophoresis and excision of the protein for mass spectrometry or sequencing; Immunoprecipitation and SDS-PAGE analysis of a protein with a molecular weight similar to the antibody's heavy or light chain; Identification of binding partners of immobilized glycoprotein Co-immunoprecipitation for determining protein-protein interactions
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Capacity (Metric): 2 to 10 μg Oxidized Antibody or Glycoprotein Coupling to 20 μL Resin
Assay: Affinity Purification
Product Line: GlycoLink™
For Use With (Application): Immunoprecipitation (IP)
Type: Immunoprecipitation/Co-Immunoprecipitation Kit
Format: Kit
Content And Storage: Sufficient For: 25 IP/Co-IP reactions, each using 20 μL antibody-coupled resin• UltraLink Hydrazide Resin, 0.5 mL• GlycoLink Coupling Buffer, 50 mL• Aniline, 0.2 mL• Sodium meta-periodate, 0.5 g• Wash Buffer, 60 mL• Zeba Spin Desalting Columns,0.5 mL, 25 columns• IP Lysis/Wash Buffer, 50 mL• PBS Pack (makes 500 mL), 1 pack• Elution Buffer, pH 2.8, 50 mL• Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL• Pierce Spin Columns-Screw Caps, 25 columns• Collection Tubes, 2 mL, 100 tubesStore at 4°C.
For Use With (Equipment): Microcentrifuge
Quantity: 1 Kit
Target: Not Target-Specific
Sufficient For: 25 Reactions
Capacity (Metric):
2 to 10 μg Oxidized Antibody or Glycoprotein Coupling to 20 μL Resin
Assay:
Affinity Purification
Product Line:
GlycoLink™
For Use With (Application):
Immunoprecipitation (IP)
Type:
Immunoprecipitation/Co-Immunoprecipitation Kit
Format:
Kit
Content And Storage:
Sufficient For: 25 IP/Co-IP reactions, each using 20 μL antibody-coupled resin• UltraLink Hydrazide Resin, 0.5 mL• GlycoLink Coupling Buffer, 50 mL• Aniline, 0.2 mL• Sodium meta-periodate, 0.5 g• Wash Buffer, 60 mL• Zeba Spin Desalting Columns,0.5 mL, 25 columns• IP Lysis/Wash Buffer, 50 mL• PBS Pack (makes 500 mL), 1 pack• Elution Buffer, pH 2.8, 50 mL• Lane Marker Sample Buffer, Non-reducing, (5X), 5 mL• Pierce Spin Columns-Screw Caps, 25 columns• Collection Tubes, 2 mL, 100 tubesStore at 4°C.
For Use With (Equipment):
Microcentrifuge
Quantity:
1 Kit
Target:
Not Target-Specific
Sufficient For:
25 Reactions
Capacity (Metric): __
Assay: __
Product Line: GlycoLink™
For Use With (Application): __
Type: __
Format: __
Content And Storage: Store at 4°C.
For Use With (Equipment): __
Quantity: 100 mL
Target: __
Sufficient For: __
Capacity (Metric):
__
Assay:
__
Product Line:
GlycoLink™
For Use With (Application):
__
Type:
__
Format:
__
Content And Storage:
Store at 4°C.
For Use With (Equipment):
__
Quantity:
100 mL
Target:
__
Sufficient For:
__
Capacity (Metric): __
Assay: __
Product Line: Pierce™
For Use With (Application): __
Type: __
Format: __
Content And Storage: Store at -20°C.
For Use With (Equipment): __
Quantity: 1000 U
Target: __
Sufficient For: __
Capacity (Metric):
__
Assay:
__
Product Line:
Pierce™
For Use With (Application):
__
Type:
__
Format:
__
Content And Storage:
Store at -20°C.
For Use With (Equipment):
__
Quantity:
1000 U
Target:
__
Sufficient For:
__
Capacity (Metric): __
Assay: __
Product Line: Pierce™
For Use With (Application): __
Type: __
Format: __
Content And Storage: Store at -20°C.
For Use With (Equipment): __
Quantity: 10,000 U
Target: __
Sufficient For: __
Capacity (Metric):
__
Assay:
__
Product Line:
Pierce™
For Use With (Application):
__
Type:
__
Format:
__
Content And Storage:
Store at -20°C.
For Use With (Equipment):
__
Quantity:
10,000 U
Target:
__
Sufficient For:
__