Thermo Scientific™ Pierce™ Crosslink Magnetic IP/Co-IP Kit

Description

• The Thermo Scientific Pierce Crosslink Magnetic IP/Co-IP Kit uses crosslinking chemistry to covalently immobilize IP antibodies onto premium-quality Protein A/G Magnetic Beads for effective immunoprecipitation and co-immunoprecipitation
• Because this magnetic IP procedure involves covalent attachment of the specific antibody used for immunoprecipitation, target proteins or co-IP protein complexes can be eluted and analyzed without antibody fragments because the antibody remains affixed to the beads
• The kit contains an optimized protocol and all buffers and reagents necessary to accomplish high-yield IP or co-IP with antibodies that bind to Protein A/G and using either manual or automated magnetic separation tools
• Highlights: Antibody-free IP – antibody is irreversibly attached to the magnetic beads resulting in negligible co-elution of intact antibody or heavy and light chains with the purified antigen Convenient – the IP/co-IP kit contains magnetic beads and all essential buffers for optimal immunoprecipitation Fast – complete crosslinking and immunoprecipitation in less than 3 hours Efficient – immunoprecipitate with half the recommended volume of magnetic particles compared to other magnetic beads Compatible – magnetic beads coupled with Protein A/G recombinant protein ensures compatibility with most primary antibodies, whether from mouse or rabbit Scalable – use only the amount of antibody needed for a single IP experiment or prepare a larger quantity of antibody-crosslinked magnetic beads for multiple experiments Includes: Pierce Protein A/G Magnetic Beads, DSS crosslinker and coupling buffer, lysis/wash buffer, elution buffer, neutralization buffer and sample loading buffer