PI26147
Thermo Scientific™ Pierce™ Crosslink IP Kit
Manufacturer: Thermo Scientific™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | PI26147-Each-of-1 | In Stock | ₹ 56,298.48 |
PI26147 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 56,298.48
GST (18%): ₹ 10,133.726
Total Price: ₹ 66,432.206
Content And Storage
Sufficient For: Preparing and performing at least 50 IP reactions, each with 10 μL of resin• Protein A/G Plus Agarose, 0.55 mL• DSS Crosslinker, 8 x 2 mg• Coupling Buffer (20X), 25 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Conditioning Buffer (100X), 5 mL• Tris-Buffered Saline (20X), 25 mL• Elution Buffer, 50 mL• Lane Marker Sample Buffer (5X), 5 mL• Control Agarose Resin, 2 mL• Spin Columns and Collection TubesStore at 4°C. Store DSS desiccated at 4°C.
For Use With (Equipment)
Microcentrifuge
Quantity
50 reactions
Target
Not Target-Specific
Sufficient For
50 Reactions
Assay
Affinity Purification
Product Line
Pierce™
For Use With (Application)
Immunoprecipitation (IP)
Type
Immunoprecipitation/Co-Immunoprecipitation Kit
Format
Kit
Related Products
Description
- Thermo Scientific™ Pierce Crosslink IP Kit is an adaptation of traditional immunoprecipitation protocols that enables antigen recovery without antibody contamination or co-elution
- This immunoprecipitation kit extends the functionality of traditional immunoprecipitation (IP) methods by adding crosslinking technology and microcentrifuge spin column sample handling to the procedure
- The primary benefits resulting from these features are the ability to purify target protein without contamination by the antibody and the ability to more effectively wash and separate samples from the beaded agarose resin
- The Pierce Crosslink IP Kit method involves capturing the IP antibody to Protein A/G Agarose resin and covalently immobilizing it to the support by crosslinking with disuccinmidyl suberate (DSS)
- The antibody resin is then incubated with the sample that contains the protein antigen of interest, allowing the antibody:antigen complex to form
- After washing to remove nonbound (presumably undesired) components of the sample, the antigen is recovered by dissociation from the antibody with elution buffer supplied in the kit
- The entire procedure is performed in a microcentrifuge spin cup, allowing solutions to be fully separated from the agarose resin upon brief centrifugation
- Only antigen is eluted by the procedure, enabling it to be identified and further analyzed without interference from antibody fragments
- Furthermore, the antibody resin often can be reused for additional rounds of immunoprecipitation
- Highlights: Eliminates antibody contamination – antibody is irreversibly attached to the agarose beads so that co-elution of heavy and light chains with the purified protein is minimized Easy and efficient scalability – use only the amount of antibody needed for a single IP experiment (e.g., 5μg) or immobilize up to 200μg of antibody to prepare a larger quantity of ready-made IP affinity resin for many experiments Assay reliability and sample handling – Pierce Spin Columns eliminate resin loss and provide for more efficient separation of solutions than traditional IP methods that use only microcentrifuge tubes Prepared antibody affinity resin is reusable – because the antibody is covalently immobilized and usually is not inactivated by the mild elution procedure, the resin often can be used several times Complete kits – package includes sufficient reagents and spin columns to perform at least 50 antibody immobilizations and IP experiments Includes: Pierce Protein A/G Plus Agarose, control agarose resin, DSS crosslinker, coupling buffer, IP lysis/wash buffer, conditioning buffer, Tris-buffered saline, elution buffer, sample loading buffer, spin columns and collection tubes Recommended for: Immunoprecipitation followed by electrophoresis and excision of the protein for mass spectrometry or sequencing identification analysis; Immunoprecipitation and SDS-PAGE analysis of a target protein whose molecular weight is similar to the heavy or light chain antibody fragment; Immunoprecipitation of target proteins for subsequent analysis by nondenaturing methods (i.e., methods not involving SDS-PAGE)
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Content And Storage: Sufficient For: Preparing and performing at least 50 IP reactions, each with 10 μL of resin• Protein A/G Plus Agarose, 0.55 mL• DSS Crosslinker, 8 x 2 mg• Coupling Buffer (20X), 25 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Conditioning Buffer (100X), 5 mL• Tris-Buffered Saline (20X), 25 mL• Elution Buffer, 50 mL• Lane Marker Sample Buffer (5X), 5 mL• Control Agarose Resin, 2 mL• Spin Columns and Collection TubesStore at 4°C. Store DSS desiccated at 4°C.
For Use With (Equipment): Microcentrifuge
Quantity: 50 reactions
Target: Not Target-Specific
Sufficient For: 50 Reactions
Assay: Affinity Purification
Product Line: Pierce™
For Use With (Application): Immunoprecipitation (IP)
Type: Immunoprecipitation/Co-Immunoprecipitation Kit
Format: Kit
Content And Storage:
Sufficient For: Preparing and performing at least 50 IP reactions, each with 10 μL of resin• Protein A/G Plus Agarose, 0.55 mL• DSS Crosslinker, 8 x 2 mg• Coupling Buffer (20X), 25 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Conditioning Buffer (100X), 5 mL• Tris-Buffered Saline (20X), 25 mL• Elution Buffer, 50 mL• Lane Marker Sample Buffer (5X), 5 mL• Control Agarose Resin, 2 mL• Spin Columns and Collection TubesStore at 4°C. Store DSS desiccated at 4°C.
For Use With (Equipment):
Microcentrifuge
Quantity:
50 reactions
Target:
Not Target-Specific
Sufficient For:
50 Reactions
Assay:
Affinity Purification
Product Line:
Pierce™
For Use With (Application):
Immunoprecipitation (IP)
Type:
Immunoprecipitation/Co-Immunoprecipitation Kit
Format:
Kit
Content And Storage: Sufficient For: Preparing and performing 50 co-IP reactions, each with 25 μL of resin• AminoLink Plus Coupling Resin, 2 mL• Coupling Buffer (20X), 25 mL• Sodium Cyanoborohydride Solution (5M), 0.5 mL• Quenching Buffer, 50 mL• Wash Solution, 60 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Modified Dulbecco's PBS (20X), 25 mL• Conditioning Buffer (100X), 5 mL• Elution Buffer, 50 mL• Lane Marker Sample Buffer (5X), 5 mL• Pierce Spin Columns, Screw Cap, 50 columns• Microcentrifuge Collection Tubes, 2 mL, 100 tubes• Microcentrifuge Collection Tubes, 1.5 mL, 50 tubes• Pierce Control Agarose Resin, 2 mLStore at 4°C.
For Use With (Equipment): Microcentrifuge
Quantity: 1 Kit
Target: Not Target-Specific
Sufficient For: 50 Reactions
Assay: Affinity Purification
Product Line: Pierce™
For Use With (Application): Immunoprecipitation (IP)
Type: Immunoprecipitation/Co-Immunoprecipitation Kit
Format: Kit
Content And Storage:
Sufficient For: Preparing and performing 50 co-IP reactions, each with 25 μL of resin• AminoLink Plus Coupling Resin, 2 mL• Coupling Buffer (20X), 25 mL• Sodium Cyanoborohydride Solution (5M), 0.5 mL• Quenching Buffer, 50 mL• Wash Solution, 60 mL• IP Lysis/Wash Buffer, 2 x 50 mL• Modified Dulbecco's PBS (20X), 25 mL• Conditioning Buffer (100X), 5 mL• Elution Buffer, 50 mL• Lane Marker Sample Buffer (5X), 5 mL• Pierce Spin Columns, Screw Cap, 50 columns• Microcentrifuge Collection Tubes, 2 mL, 100 tubes• Microcentrifuge Collection Tubes, 1.5 mL, 50 tubes• Pierce Control Agarose Resin, 2 mLStore at 4°C.
For Use With (Equipment):
Microcentrifuge
Quantity:
1 Kit
Target:
Not Target-Specific
Sufficient For:
50 Reactions
Assay:
Affinity Purification
Product Line:
Pierce™
For Use With (Application):
Immunoprecipitation (IP)
Type:
Immunoprecipitation/Co-Immunoprecipitation Kit
Format:
Kit
Content And Storage: Store at 2–8°C.
For Use With (Equipment): __
Quantity: 10 mL
Target: __
Sufficient For: __
Assay: __
Product Line: Pierce™
For Use With (Application): __
Type: Resin
Format: Bottle
Content And Storage:
Store at 2–8°C.
For Use With (Equipment):
__
Quantity:
10 mL
Target:
__
Sufficient For:
__
Assay:
__
Product Line:
Pierce™
For Use With (Application):
__
Type:
Resin
Format:
Bottle
Content And Storage: • Glycine Solution (10X), 15 mL; store at room temperature• PBS (20X), 15 mL; store at 4°C• Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 1 mL; store at 4°C• Membrane Extraction Buffer, 10 mL; store at 4°C• MNase Digestion Buffer, 10 mL; store at 4°C• DTT (7.7 mg), Lyophilized, 2 vials; store at room temperature or 4°C• MNase Stop Solution, 1 mL; store at 4°C• ChIP-Grade Protein A/G Magnetic Beads, 0.7mL; store at 4°C• IP Dilution/Wash Buffer (5X), 40 mL, store at 4°C• Sodium Chloride (5M), 6 mL; store at 4°C• IP Elution Buffer (2X), 4.5 mL; store at 4°C• Microcentrifuge Tubes, 1.5 mL, 75 tubes; store at room temperature or 4°C• DNA Clean-Up Column and Reagents, 40 purifications; store at room temperature or 4°C• DNA Clean-Up Column, 40 columns• DNA Column Binding Solution, 30 mL• DNA Column Wash Solution, 6 mL• pH Indicator, 0.8 mL• DNA Column Elution Solution, 5 mL• Micrococcal Nuclease (ChIP Grade) (10U/μL), 50 μL; store at -20°C• Anti-RNA Polymerase II Antibody, 25 μL; store at -20°C• Normal Rabbit IgG (1mg/mL), 10 μL; store at -20°C• Proteinase K (20mg/mL), 0.25 mL; store at -20°C• ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL; store at -20°C
For Use With (Equipment): __
Quantity: 30 reactions
Target: __
Sufficient For: 30 Reactions
Assay: __
Product Line: Pierce™
For Use With (Application): __
Type: ChIP Kit
Format: Kit
Content And Storage:
• Glycine Solution (10X), 15 mL; store at room temperature• PBS (20X), 15 mL; store at 4°C• Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X), 1 mL; store at 4°C• Membrane Extraction Buffer, 10 mL; store at 4°C• MNase Digestion Buffer, 10 mL; store at 4°C• DTT (7.7 mg), Lyophilized, 2 vials; store at room temperature or 4°C• MNase Stop Solution, 1 mL; store at 4°C• ChIP-Grade Protein A/G Magnetic Beads, 0.7mL; store at 4°C• IP Dilution/Wash Buffer (5X), 40 mL, store at 4°C• Sodium Chloride (5M), 6 mL; store at 4°C• IP Elution Buffer (2X), 4.5 mL; store at 4°C• Microcentrifuge Tubes, 1.5 mL, 75 tubes; store at room temperature or 4°C• DNA Clean-Up Column and Reagents, 40 purifications; store at room temperature or 4°C• DNA Clean-Up Column, 40 columns• DNA Column Binding Solution, 30 mL• DNA Column Wash Solution, 6 mL• pH Indicator, 0.8 mL• DNA Column Elution Solution, 5 mL• Micrococcal Nuclease (ChIP Grade) (10U/μL), 50 μL; store at -20°C• Anti-RNA Polymerase II Antibody, 25 μL; store at -20°C• Normal Rabbit IgG (1mg/mL), 10 μL; store at -20°C• Proteinase K (20mg/mL), 0.25 mL; store at -20°C• ChIP Positive Control Primers (GAPDH promoter, Human-specific), 100 μL; store at -20°C
For Use With (Equipment):
__
Quantity:
30 reactions
Target:
__
Sufficient For:
30 Reactions
Assay:
__
Product Line:
Pierce™
For Use With (Application):
__
Type:
ChIP Kit
Format:
Kit