Thermo Scientific™ Pierce™ Ubiquitin Enrichment Kit

Description

• Thermo Scientific™ Pierce Ubiquitin Enrichment Kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples
• The kit uses anti-ubiquitin affinity resin to bind and purify polyubiquitinated proteins (i.e., those with modifications containing more than four ubiquitin monomers) from cell lysates and other protein samples
• The enriched fraction can subsequently be analyzed to determine the amount of general ubiquitin conjugates present or to identify a specific protein of interest by Western blotting
• The assay protocol is fast and straight-forward, allowing for isolation of polyubiquitinated proteins and the fractionation of monoubiquitinated species in the resin flow-through
• The Ubiquitin Enrichment Kit outperforms kits from other suppliers and provides a clean and specific preparation of proteins when compared to a control resin
• Highlights: Fast – less than 45 minutes hands-on time Complete – includes all reagents needed for ubiquitin-modified protein enrichment from cultured cells and tissue samples, as well as anti-ubiquitin antibody serum for Western blot analysis Flexible – sample incubation from two hours to overnight allows assay to be completed in several hours or in less than 30 minutes after overnight incubation Robust – compatible with all Thermo Scientific Pierce Cell Lysis Products and standard RIPA formulations Multiple-sample format – easily processes 1 to 15 samples concurrently Includes: Polyubiquitin affinity resin (300μL), Tris-buffered saline pack, microcentrifuge spin columns and accessories, polyubiquitin positive control and anti-ubiquitin rabbit anti-serum