Thermo Scientific™ His-Tag Magnetic Agarose Purification Bundle

Description

• Pierce Ni-NTA Magnetic Agarose Beads consist of highly crosslinked agarose beads embedded with magnetite and a covalently attached tetradentate nitrilotriacetic acid (NTA) chelator charged with divalent nickel ions
• The density of the ligand on the magnetic agarose bead results in a binding capacity similar to or better than traditional agarose resins with the added feature of magnetic handling Magnetic agarose beads are a valuable tool for small-scale purification of multiple His-tagged proteins and for scouting expression and purification conditions to be used in larger-scale purifications with agarose chromatography supports Thermo Scientific B-PER Complete Bacterial Protein Extraction Reagent is an easy-to-use cell lysis reagent
• It is a nonionic detergent-based solution that effectively disrupts cells and solubilizes native or recombinant proteins without denaturation
• B-PER Complete Bacterial Protein Extraction Reagent is an all-inclusive formulation that combines the lysis reagent with lysozyme and a universal nuclease to enable mild extraction from both gram-negative and gram-positive bacteria Thermo Scientific Halt Protease Inhibitor Cocktail combined with B-PER added before use, protects proteins from proteolytic degradation through inhibition of serine proteases, cysteine proteases, aspartic acid proteases, and aminopeptidases that are typically present in cell lysate samples